Beckman Coulter MicroScan B1016-138 Manuel D'utilisation page 6

H. For wells containing the aminoglycosides (e.g., gentamicin) and macrolides (e.g., erythromycin), growth may not
be as heavy as that in the growth control well due to differences in basal media. Care should be taken in interpreting
these results.
I. A "trailing
trimethoprim/sulfamethoxazole (T/S) with the use of the RENOK Rehydrating/Inoculating System is due to the
inoculum concentration. The end point should be read as the lowest concentration which when compared to the
growth well shows:
a. Approximately 80% reduction of growth (T/S)
b. A white button which is less than 2mm in diameter or
c. A white button which is semi-translucent.
J. A slight haze may be observed with the fluoroquinolone class of antimicrobics (e.g., ciprofloxacin, norfloxacin,
ofloxacin) when using the Prompt method of inoculation and staphylococci, including Quality Control organism S.
aureus ATCC 29213. This should NOT be interpreted as growth.
K. If an organism does not grow in the Thymidine Free Growth (TFG) well, an MIC should not be reported for T/S.
6. Reading Identification Substrates
A. Read all identification substrates with a white background except CV, MS, NOV, OPT, NACL, and BAC, which
should be read against a black (indirectly lighted) background.
B. IDX and PHO results are recorded after 16-20 hours incubation.
C. Panels with negative PGT and fewer than 3 positive carbohydrates should be reincubated for an additional 24
hours before making a final reading and identification.
D. Only panels which have 3 carbohydrates or the PGT reaction positive should have reagents added at 16-20 hours.
At 40-44 hours, add reagents regardless of number of positive tests.
E. Prior to addition of reagents, record all positive reactions.
F. Add reagents as follows:
1) Add 1 drop 40% Potassium Hydroxide (KOH) and 1 drop of 5% Alpha Naphthol to the VP well. Wait at least 20
minutes for VP reaction to develop prior to reading.
2) Add 1 drop each of 0.8% Sulfanilic Acid and 0.5% N, N-Dimethyl-alpha-naphthylamine to the NIT well. Wait at
least 5 minutes for the NIT reaction to develop prior to reading.
3) Add 2 drops of Peptidase reagent to PYR well. Wait 2 minutes prior to reading.
G. Refer to RESULTS section for aid in biochemical interpretation.
RESULTS
1. Biochemical Results
A. Biochemical Interpretations
Well
CV
MS
NOV
OPT
NACL
BAC
NIT
PGR
PHO
PGT
IDX
VP
BE
PYR
ARG
URE
C29870–AD
effect" may be observed
Reagent
Add 1 drop 0.8% Sulfanilic Acid and 1 drop of 0.5%
N, N-Dimethyl-alpha-naphthylamine. Wait 5 minutes for the reaction to develop.
Any shade of yellow should be interpreted
as positive. Use the NOV well as a negative
control.
Add 1 drop of 40% KOH and 1 drop of 5% Alpha Naphthol. Wait at least 20
minutes for the reaction to develop.
Add 2 drops Peptidase reagent. Wait 2 minutes for the reaction to develop.
in some drug/organism
2
6 of 339
combinations. Trailing
Positive Negative
Growth No Growth
Pink to Red Clear (colorless)
may be very Pale
Pink
Yellow Clear (colorless)
Blue to Gray/ Clear (colorless) or
precipitate White precipitate
Pink to Red Clear (colorless)
to Brown, may be
cloudy or very Pale
Pink
Dark Brown to Clear (colorless) to
Black Light Brown
Red/Orange to Red Yellow to
Orange/Red
Pink/Orange to Yellow to Orange
Pink
Pink Yellow to Orange
with