Beckman Coulter MicroScan B1016-138 Manuel D'utilisation page 12

Organism
L. monocytogenes
Staphylococci
Coagulase-Negative
Staphylococci
Coagulase-Negative
Methicillin Resistant
Staphylococci
Methicillin Resistant
S. saprophyticus
S. agalactiae
(Group B)
S. bovis group
S. haemolyticus
1. When printing is not suppressed by LabPro, manually suppress the result. Please consult the LabPro Operator's Guide for instructions.
2. For breakpoint dilutions only.
3. Limitation is only applicable when using panels without a 16 μg/mL dilution.
11. Vancomycin-resistant E. faecium may be distinguished from E. gallinarum by checking motility and pigment production
(refer to the Additional Test Table in the Microbiologics Manual).
12. The QC Range for some antimicrobial agents do not agree with the CLSI Acceptable Quality Control Ranges listed in
the CLSI Document. Refer to the Quality Control table for specific information.
13. The Prompt System demonstrated elevated MICs with fluoroquinolones (e.g., ciprofloxacin), lincosamides (e.g.,
clindamycin) and macrolides (e.g., erythromycin) with staphylococci when compared with the Reference method.
Inoculum concentration is critical with these antimicrobic organism combinations. If an Intermediate or Resistant
interpretation is obtained using the Prompt System, an alternate method of obtaining a susceptibility result should be
used (e.g., turbidity using a non-breakpoint format) prior to reporting results.
14. Broth and agar dilution tests may not accurately detect penicillin and ampicillin resistance with β-lactamase producing
enterococci strains.
15. The ability of the MicroScan Dried Gram Positive Panels to detect resistance to meropenem among L. monocytogenes
is unknown because resistant strains were not available at the time of comparative testing.
16. The ability of the MicroScan Dried Gram Positive Panels to detect resistance to linezolid and ceftaroline is unknown
because resistant strains were not available at the time of comparative testing.
17. It is important to speciate strains of Enterococcus since Synercid is not active against E. faecalis.
18. autoSCAN-4 and WalkAway instrument results obtained with S. saprophyticus and mupirocin have shown discrepant
MICs when compared with a manual read of the panels. MIC results for these isolates should be obtained by reading
panels manually.
19. MicroScan Dried Gram Positive Panels detected vancomycin resistance in the VRSA S. aureus strains available at the
time of comparative testing. The ability of the MicroScan Dried Gram Positive Panels to detect vancomycin resistance
in other S. aureus strains is unknown due to the limited number of resistant strains available for comparative testing.
20. autoSCAN-4 instrument results obtained with vancomycin and staphylococci showed a potential for light growth at 16
hour incubation. Vancomycin results with staphylococci should be read on the autoSCAN-4 instrument after 18-20 hour
incubation.
21. The Prompt System demonstrated elevated daptomycin MICs with staphylococci when compared with the reference
method. If a nonsusceptible interpretation is obtained using the Prompt System, an alternate method of obtaining a
susceptible result should be used (e.g., turbidity) prior to reporting.
22. The Prompt System should not be used when the colony size is smaller than the wand tip. Examples of organisms
that may not meet the size requirement are Streptococcus spp. other than S. bovis group and S. agalactiae (Group B).
C29870–AD
Drugs which, if critical
to patient care, alternate
method should be used
Drugs which will not be reported
Arbekacin
Azithromycin
Ceftaroline
Clarithromycin
Ertapenem
Erythromycin
Meropenem
Trimethoprim/Sulfamethoxazole
Teicoplanin (EUCAST Only)
Ertapenem
Piperacillin/Tazobactam
Mupirocin
Amikacin
Kanamycin
Tobramycin
Cefotaxime (EUCAST Only)
Teicoplanin 3
(CLSI only)
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