Intended Use; Warning And Precautions - Beckman Coulter MicroScan B1016-138 Manuel D'utilisation

MicroScan
Gram Positive Procedural Manual, Multi-regional PU-05 and older panels
B1016-138, B1016-139, B1016-140,
B1016-145, B1016-146, B1016-152,
B1016-160, B1016-161, B1016-164,
B1016-165, B1016-167, B1016-172,
B1016-173, B1016-174

INTENDED USE

For use with MicroScan Dried Gram Positive MIC/Combo and Dried Gram Positive Breakpoint Combo and Dried Gram Positive
ID Type 2 or 3 panels. MicroScan Pos panels are designed for use in determining antimicrobial agent susceptibility and/or
identification to the species level of rapidly growing aerobic and facultative gram positive cocci, some fastidious aerobic gram
positive cocci and Listeria monocytogenes. Refer to Limitations of Procedure Section for use with fastidious streptococci.
SUMMARY AND PRINCIPLE
The antimicrobial susceptibility tests are miniaturizations of the broth dilution susceptibility test which have been dehydrated.
Various antimicrobial agents are diluted in Mueller-Hinton broth with calcium and magnesium, or Mueller-Hinton broth with
additional supplementation to concentrations bridging the range of clinical interest.
sodium chloride. 1 The synergy screens utilize dextrose phosphate broth. The MicroScan Inducible Clindamycin test is
intended to detect inducible resistance for staphylococci with the antimicrobial agent clindamycin.
The MicroScan Cefoxitin Screen is intended to determine the susceptibility of Staphylococcus species to the
penicillinase-stable beta-lactams. The Cefoxitin Screen uses the 16-20 hour result from a well containing cefoxitin at 4 μg/mL
and growth media, labeled CfxS, and the oxacillin MIC at 16-20 hours.
After inoculation and rehydration with a standardized suspension of organism and incubation at 35°C for 16-20 hours*,
the minimum inhibitory concentration (MIC) or a qualitative susceptibility (Susceptible, Intermediate or Resistant) for
the test organism is determined by observing the lowest antimicrobial concentration showing inhibition of growth.
2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22
Modified conventional and chromogenic tests are used for the identification of Micrococcaceae, Streptococcaceae, and Listeria
monocytogenes. Identification is based on detection of pH changes, substrate utilization, and growth in the presence of
antimicrobial agents after 16-44 hours incubation at 35°C.
* Accurate detection of resistance requires extended incubation times for the following organism/antimicrobics:
24 hours
24-48 hours
1. 24 hour incubation is not required for Staphylococcus species for panels containing the Cefoxitin Screen Well.
Reagents
Identification Substrates
Crystal Violet
Micrococcus Screen
Nitrate
Novobiocin
PNP-β-D-Glucuronide
Indoxyl Phosphatase
Voges-Proskauer
Optochin
Phosphatase
40% Bile Esculin
L-Pyrrolidonyl-β-naphthylamide
Arginine
PNP-β-D-Galactopyranoside
Urea

WARNING AND PRECAUTIONS

1. For in vitro diagnostic use only.
2. Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures, with
special awareness that the inoculated panels contain potentially pathogenic organisms.
3. This material contains infectious agents and should be discarded as appropriate for biohazardous waste.
4. Results of this test should always be interpreted in conjunction with the patient's medical history, clinical presentation
and other findings.
C29870–AD
Enterococci
Staphylococci
Streptococci
Enterococci
Abbr.
CV
MS
NIT
NOV
PGR
IDX
VP
OPT
PHO
BE
PYR
ARG
PGT
URE
23,24,25,26,27,28,29
Kanamycin Synergy Screen
1
Oxacillin
Kanamycin Synergy Screen
Streptomycin Synergy Screen
Identification Substrates
Mannitol
Lactose
Trehalose
Mannose
Sodium Chloride 6.5%
Sorbitol
Arabinose
Ribose
Inulin
Raffinose
Bacitracin
Pyruvate
Thymidine-Free Growth Well
2 of 339
Oxacillin broth is supplemented with
1
Abbr.
MAN
LAC
TRE
MNS
NACL
SOR
ARA
RBS
INU
RAF
BAC
PRV
TFG