Beckman Coulter MicroScan Manuel D'utilisation page 2

MicroScan
Rapid Yeast ID Procedural Manual
B1017-70
INTENDED USE
The Rapid Yeast Identification Panel is designed for rapid identification of yeast and yeast-like species isolated from clinical
specimens.
SUMMARY AND PRINCIPLE
Chromogenic and modified conventional tests are used for the identification of yeast species isolated from clinical specimens.
The Rapid Yeast Identification Panel is a 96 well microdilution panel that utilizes 27 dehydrated substrates (Refer to Table
1-1). A heavy suspension of the yeast in autoclaved water is used to rehydrate the substrates. An identification is obtained
after incubation of the panel at 35-37°C for 4 hours.
WARNING AND PRECAUTIONS
1. Rapid Yeast Identification Panels are for in vitro diagnostic use only.
2. Do not allow Peptidase or Sodium Hydroxide reagent or any of the identification substrates to come in contact with eyes,
skin, or clothing.
3. Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures, with
awareness that inoculated panels contain potentially pathogenic organisms.
4. All materials should be autoclaved prior to disposal.
STORAGE
Store panels and Peptidase reagent at 2-8°C.
GHS HAZARD CLASSIFICATION
Not classified as hazardous
Safety Data Sheet is available at techdocs.beckmancoulter.com
SPECIMEN COLLECTION AND PREPARATION
Refer to the American Society for Microbiology Manual of Clinical Microbiology
recommended procedures for the transport and isolation of yeasts. Rapid chromogenic tests are based upon the presence of
preformed enzymes in the unknown organism.
test results because of the induction of different enzymes by different media. The Rapid Yeast Identification Panel is based
upon the use of Sabouraud Dextrose Agar (either standard or Emmons' modification). The presence of antibiotics in the
growth medium is not a critical variable, however media containing blood, Yeast Extract-Malt Extract (YM) Agar and Inhibitory
Mold Agar (IMA) should not be used.
MATERIALS PROVIDED
Rapid Yeast Identification Panels (B1017-70)
Worksheet
Procedural Manual
Quality Control Chart
MATERIALS REQUIRED, BUT NOT PROVIDED
Inoculum Water (B1015-2)
0.05N Sodium Hydroxide (B1015-3)
Peptidase Reagent (B1012-30B)
Yeast Turbidity standard (B1015-18)
Cover Trays (B1010-56B)
MicroScan Yeast Biotype Codebook (B1014-1A)
Inoculating loops
Sabouraud Dextrose Agar plates
Vortex mixer
50 μL pipettor with tips
Incubator (35-37°C), non-CO
Quality control organisms:
C29879–AB
Thus, the plating medium on which the organism is grown will influence the
2,3,4
2
or other mycology reference manuals for
1
2 of 141