3M Molecular Detection Assay 2 Manuel D'instructions page 9

Preparation of the 3M™ Molecular Detection Chill Block Insert
Place the 3M Molecular Detection Chill Block directly on the laboratory bench; (the 3M™ Molecular Detection Chill Block
Tray is not used). Use the chill block at ambient laboratory temperature (20-25°C).
Preparation of the 3M™ Molecular Detection Heat Block Insert
Place the 3M Molecular Detection Heat Block Insert in a dry double block heater unit. Turn on the dry block heater unit and
set the temperature to allow the 3M Molecular Detection Heat Block Insert to reach and maintain a temperature of
100 ±1°C.
Note: Depending on the heater unit, allow approximately 30 minutes for the 3M Molecular Detection Heat Block Insert
to reach temperature. Using an appropriate, calibrated thermometer (e.g., a partial immersion thermometer or digital
thermocouple thermometer, not a total immersion thermometer) placed in the designated location, verify that the 3M
Molecular Detection Heat Block Insert is at 100 ±1°C.
Preparation of the 3M Molecular Detection Instrument
1. Launch the 3M™ Molecular Detection Software and log in. Contact your 3M Food Safety representative to ensure you
have the most updated version of the software.
2. Turn on the 3M Molecular Detection Instrument.
3. Create or edit a run with data for each sample. Refer to the 3M Molecular Detection System User Manual for details.
Note: The 3M Molecular Detection Instrument must reach and maintain temperature of 60°C before inserting the 3M
Molecular Detection Speed Loader Tray with reaction tubes. This heating step takes approximately 20 minutes and is
indicated by an ORANGE light on the instrument's status bar. When the instrument is ready to start a run, the status bar
will turn GREEN.
Lysis
1. Allow the lysis solution (LS) tubes to warm up by setting the rack at room temperature (20-25°C) overnight (16-18
hours). Alternatives to equilibrate the LS tubes to room temperature are to set the LS tubes on the laboratory bench for
at least 2 hours, incubate the LS tubes in a 37 ±1°C incubator for 1 hour or place them in a dry double block heater for 30
seconds at 100°C.
2. Invert the capped tubes to mix. Proceed to next step within 4 hours.
3. Remove the enrichment broth from the incubator.
4. One LS tube is required for each sample and the Negative Control (NC) (sterile enrichment medium) sample.
4.1 LS tube strips can be cut to desired LS tube number. Select the number of individual LS tubes or 8-tube strips
needed. Place the LS tubes in an empty rack.
4.2 To avoid cross-contamination, decap one LS tubes strip at a time and use a new pipette tip for each transfer step.
4.3 Transfer enriched sample to LS tubes as described below:
Transfer each enriched sample into an individual LS tube first. Transfer the NC last.
4.4 Use the 3M™ Molecular Detection Cap/Decap Tool-Lysis to decap one LS tube strip – one strip at a time.
4.5 Discard the LS tube cap – if lysate will be retained for retest, place the caps into a clean container for
re-application after lysis. For processing of retained lysate, see Appendix A.
4.6 Transfer 20 µL of sample into a LS tube unless otherwise indicated in Protocol Tables 2, 3, and 4 (e.g., raw dairy
products use 10 µL).
5. Repeat step 4.2 until each individual sample has been added to a corresponding LS tube in the strip.
6. Repeat steps 4.1 to 4.6 as needed, for the number of samples to be tested.
7. When all samples have been transferred, transfer 20 µL of NC (sterile enrichment medium, e.g., Demi-Fraser Broth) into
a LS tube. Do not use water as a NC.
8. Verify that the temperature of the 3M Molecular Detection Heat Block Insert is at 100 ±1°C.
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EN (English)
20 µl