3M Molecular Detection Assay 2 Manuel D'instructions page 7

Table 3. Enrichment protocols using Demi-Fraser Broth
Performance Tested Certificate #111501
SM
Sample Matrix
Beef hot dogs, Queso Fresco, Vanilla Ice
Cream, 4% Milk Fat Cottage Cheese,
3% chocolate whole milk, romaine
lettuce, bagged raw spinach, cold
smoked salmon
Raw chicken
Deli turkey
Cantaloupe
(b)
Stainless steel
Sealed concrete
Plastic
All samples for the AOAC validation were homogenized by stomaching unless otherwise noted.
(a) Demi-Fraser and Fraser broth should always be supplemented with Fraser Broth supplement (ferric ammonium citrate)
during primary or secondary enrichment.
(b) Homogenize sample by hand mixing.
(c) Homogenize sample by vortexing.
NF Validation by AFNOR Certification
For more information about end of validity, please refer to NF Validation certificate available on the website mentioned
above.
NF Validation certified method in compliance with ISO 16140-2
Scope of the validation: All human food and environmental samples (excluding primary production samples)
Sample preparation: Samples should be prepared according to EN ISO 11290-1
Software version: See certificate
(a)
Sample Size
25 g
25 g
125 g
Whole melon
1 sponge
1 sponge
1 swab
(c)
3M 01/14-05/16
Alternative Analytical Methods for Agribusiness
http:/ /nf-validation.afnor.org/en
at 37 ± 1°C according to AOAC Official Methods
Enrichment Broth Volume (mL)
225
475
1125
Enough volume to allow melon to float
225
100
10
in comparison to ISO 11290-1
(8)
and EN ISO 6887
(3)
7
EN (English)
2016.07 and
SM
Enrichment Time (hr)
24-30
28-32
24-30
26-30
24-30
24-30
24-30
(3)
(9)