3M Molecular Detection Assay 2 Manuel D'instructions page 2

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Molecular Detection Assay 2 - Listeria
Product Description and Intended Use
3M™ Molecular Detection Assay 2 - Listeria is used with the 3M™ Molecular Detection System for the rapid and specific
detection of Listeria species in enriched food and environmental samples.
The 3M Molecular Detection Assays use loop-mediated isothermal amplification to rapidly amplify nucleic acid sequences
with high specificity and sensitivity, combined with bioluminescence to detect the amplification. Presumptive positive
results are reported in real-time while negative results are displayed after the assay is completed. Presumptive positive
results should be confirmed using your preferred method or as specified by local regulations
The 3M Molecular Detection Assay 2 - Listeria is intended for use in a laboratory environment by professionals trained
in laboratory techniques. 3M has not documented the use of this product in industries other than food or beverage. For
example, 3M has not documented this product for testing water, pharmaceutical, cosmetics, clinical or veterinary samples.
The 3M Molecular Detection Assay 2 - Listeria has not been evaluated with all possible testing protocols or with all
possible strains of bacteria.
As with all test methods, the source, formulation and quality of enrichment medium can influence the results. Factors
such as sampling methods, testing protocols, sample preparation, handling, and laboratory technique may also influence
results. 3M recommends evaluation of the method including enrichment medium, in the user's environment using a
sufficient number of samples with particular foods and/or environmental samples and microbial challenges to ensure that
the method meets the user's criteria.
3M has evaluated the 3M Molecular Detection Assay 2 - Listeria with Demi-Fraser Broth and Fraser Broth containing Ferric
Ammonium Citrate. A typical formulation of these media follows below.
Demi-Fraser Broth Base Typical Formula (g/L)
Sodium Chloride
Sodium Phosphate, dibasic, anhydrous *
Beef Extract
Pancreatic Digest of Casein
Peptic Digest of Animal Tissue
Yeast Extract
Lithium Chloride
Potassium Phosphate, monobasic
Esculin
Acriflavin HCl
Nalidixic Acid
* Substitute: Sodium Phosphate, dibasic, dihydrate
Fraser Broth Supplement
(Ingredients per 10 mL vial. One vial is added to one liter of basal medium.)
Ferric Ammonium Citrate
Final pH 7.2 ± 0.2 at 25°C
The 3M™ Molecular Detection Instrument is intended for use with samples that have undergone heat treatment during
the assay lysis step, which is designed to destroy organisms present in the sample. Samples that have not been properly
heat treated during the assay lysis step may be considered a potential biohazard and should NOT be inserted into the 3M
Molecular Detection Instrument.
3M Food Safety is certified to ISO (International Organization for Standardization) 9001 for design and manufacturing.
The 3M Molecular Detection Assay 2 - Listeria test kit contains 96 tests, described in Table 1.
Fraser Broth Base Typical Formula (g/L)
20 g Sodium Chloride
9.6 g Sodium Phosphate, dibasic, anhydrous
5.0 g Beef Extract
5.0 g Pancreatic Digest of Casein
5.0 g Peptic Digest of Animal Tissue
5.0 g Yeast Extract
3.0 g Lithium Chloride
1.35 g Potassium Phosphate, monobasic
1.0 g Esculin
0.0125 g Acriflavin HCl
0.01 g Nalidixic Acid
12.0 g
0.5 g/10 mL
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EN (English)
Issue Date: 2016-08
Product Instructions
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(1, 2, 3)
20 g
9.6 g
5.0 g
5.0 g
5.0 g
5.0 g
3.0 g
1.35 g
1.0 g
0.025 g
0.02 g