3M Molecular Detection Assay 2 Manuel D'instructions page 8

Table 4. Enrichment protocols according to NF VALIDATION certified method 3M 01/14-05/16.
Enrichment
General Sample Broth
Protocol Size Volume
(mL)
All food
samples
(excepted
raw
meats,
25 g 225
raw
seafood
and raw
dairy
products)
25 g,
Environ-
1 swab,
mental 225
or 1
samples
wipe
Enrichment
Specific Sample Broth
Protocol 1 Size Volume
(mL)
Raw
meats
25 g 475
and raw
seafood
Primary Enrichment (Demi-Fraser Broth)
Enrichment
Specific
Sample Broth
Protocol 2
Size Volume
(mL)
Raw dairy
25 g 225
products
(a) Volume of sample transferred to Lysis Solution tubes. Refer to Step 4.6 of Lysis section.
(b) Demi-Fraser and Fraser broth should always be supplemented with Fraser Broth supplement (ferric ammonium citrate)
during primary or secondary enrichment.
(c) Volume of sample transferred to Lysis Solution tubes. Refer to Step 4.6 of Lysis section.
Note: Samples larger than 25 g have not been tested in the NF validation study.
Preparation of the 3M™ Molecular Detection Speed Loader Tray
1. Wet a cloth with a 1-5% (v:v in water) household bleach solution and wipe the 3M Molecular Detection Speed Loader
Tray.
2. Rinse the 3M Molecular Detection Speed Loader Tray with water.
3. Use a disposable towel to wipe the 3M Molecular Detection Speed Loader Tray dry.
4. Ensure the 3M Molecular Detection Speed Loader Tray is dry before use.
Enrichment
Tempera- Enrichment
ture Time (hr)
Volume
(±1°C)
37 24-30
37 24-30
Enrichment
Tempera- Enrichment
ture Time (hr) Volume
(±1°C)
37 28-32
(b)
Enrichment
Tempera- Enrichment
ture Time (hr)
(±1°C)
37 20-24
8
Recom-
Sample
mended
Analysis
Interruption
(a)
Point
• DF broth
up to
72 hr
• lysate at
20 µL
-20°C
• lysate at
4°C up to
72 hr
• lysate at
20 µL
-20°C
Sample Recom-
Analysis mended
Interruption
(a)
(µL) Point
• DF broth
up to
72 hr
• lysate at
20 µL
-20°C
• lysate at
4°C up to
72 hr
Secondary Enrichment (Fraser Broth)
Enrichment
Sample Tempera- Enrichment
Size ture Time (hr)
(±1°C)
Tranfer
0.1 mL
into
37
10 mL
Fraser
Broth
EN (English)
(b)
Recom-
Sample
mended
Analysis
Interruption
Volume
(c)
Point
• DF broth
up to
20-24 10 µL 72 hr
• lysate at
-20°C