3M Molecular Detection Assay 2 Manuel D'instructions page 6

Table 2: General enrichment protocols using Demi-Fraser Broth Enrichment at 37 ±1°C and Fraser Broth as needed.
Sample Sample
Matrix Size
Heat-
processed,
cooked,
cured meats,
poultry,
seafood and
fish
Heat-
25 g
processed /
pasteurized
dairy products
Produce and
vegetables
Multi-
component
foods
1 sponge
Environmental
samples
1 swab
Raw meat,
poultry, 25 g
seafood, fish
Primary Enrichment (Demi-Fraser Broth)
Sample
Matrix
Sample
Size
Raw dairy
25 g
products
(a) Demi-Fraser and Fraser broth should always be supplemented with Fraser Broth supplement (ferric ammonium citrate)
during primary or secondary enrichment.
(b) Volume of sample transferred to Lysis Solution tubes. Refer to Step 4.6 of Lysis section.
Specific Instructions for Validated Methods
AOAC® Official Method
SM
AOAC® Performance Tested Method
In AOAC OMA and PTM studies, the 3M Molecular Detection Assay 2 - Listeria was found to be an effective method for
the detection of Listeria species. The matrices tested in the study are shown in Table 3.
Enrichment
Enrichment
Broth
Temperature
Volume
(±1°C)
(mL)
225 37
100 or 225 37
10 37
475 37
Enrichment
Enrichment
Broth
Temperature
Volume
(±1°C)
(mL)
225 37
2016.07
#111501
SM
Enrichment
Time (hr)
24-30
24-30
24-30
28-32
Secondary Enrichment (Fraser
(a)
Enrichment
Enrichment Sample
Temperature
Time (hr) Size
Transfer
0.1 mL
into
20-24
10 mL
Fraser
Broth
6
EN (English)
Broth)
(a)
Analysis
Volume
Enrichment
Time (hr)
(±1°C)
37 20-24
Sample
(b)
10 μL