3M MDA2CAM96 Mode D'emploi page 9

9. Remove the uncovered rack of 3M Lysis Solution tubes from the 3M Molecular Detection Heat Block and allow to cool
in the 3M Molecular Detection Chill Block Insert at least 5 minutes and a maximum of 10 minutes. The 3M Molecular
Detection Chill Block Insert, used at ambient temperature without the 3M™ Molecular Detection Chill Block Tray,
should sit directly on the laboratory bench. When cool, the 3M Lysis Solution will revert to a pink color.
10. Remove the rack of 3M Lysis Solution tubes from the 3M Molecular Detection Chill Block Insert.
Amplification
1. One 3M Molecular Detection Assay 2 - Campylobacter Reagent Tube is required for each sample and the NC.
1.1 Tube strips can be cut to desired tube number. Select the number of individual 3M Molecular Detection Assay 2 -
Campylobacter Reagent Tube or 8-tube strips needed.
1.2 Place tubes in an empty rack.
1.3 Avoid disturbing the reagent pellets from the bottom of the tubes.
2. Select one 3M Reagent Control Tube and place in rack.
3. To avoid cross-contamination, decap one 3M Molecular Detection Assay 2 - Campylobacter Reagent Tube strip at a
time and use a new pipette tip for each transfer step.
4. Transfer each of the lysate to a 3M Molecular Detection Assay 2 - Campylobacter Reagent Tube and 3M Reagent
Control Tube as described below:
Transfer each sample lysate into individual 3M Molecular Detection Assay 2 - Campylobacter Reagent Tube first
followed by the NC. Hydrate the 3M Reagent Control Tube last.
5. Use the 3M™ Molecular Detection Cap/Decap Tool-Reagent to decap the 3M Molecular Detection Assay 2 -
Campylobacter Reagent Tubes - one strip at a time. Discard cap.
5.1 Transfer 20 µL of sample lysate from the upper ½ of the liquid (avoid precipitate) in the 3M Lysis Solution Tube
into corresponding 3M Molecular Detection Assay 2 - Campylobacter Reagent Tube. Dispense at an angle to
avoid disturbing the pellets. Mix by gently pipetting up and down 5 times.
5.2 Repeat step 5.1 until individual sample lysate has been added to a corresponding 3M Molecular Detection Assay
2 - Campylobacter Reagent Tube in the strip.
5.3 Cover the 3M Molecular Detection Assay 2 - Campylobacter Reagent Tubes with the provided extra caps and use
the rounded side of the 3M Molecular Detection Cap/Decap Tool-Reagent to apply pressure in a back and forth
motion ensuring that the cap is tightly applied.
5.4 Repeat steps 5.1 to 5.3 as needed, for the number of samples to be tested.
5.5 When all sample lysates have been transferred, repeat 5.1 to 5.3 to transfer 20 µL of NC lysate into a 3M Molecular
Detection Assay 2 - Campylobacter Reagent Tube.
5.6 Transfer 20 µL of NC lysate into a 3M Reagent Control Tube. Dispense at an angle to avoid disturbing the pellets.
Mix by gently pipetting up and down 5 times.
6. Load capped tubes into a clean and decontaminated 3M Molecular Detection Speed Loader Tray. Close and latch the
3M Molecular Detection Speed Loader Tray lid.
7. Review and confirm the configured run in the 3M Molecular Detection Software.
8. Click the Start button in the software and select instrument for use. The selected instrument's lid automatically opens.
00:15:00
99-101ºC
00:05:00
20 µL
8
EN (English)
20-25ºC