3M MDA2CAM96 Mode D'emploi page 3

WARNING: Indicates a hazardous situation, which, if not avoided, could result in death or serious injury and/or
property damage.
NOTICE: Indicates a potentially hazardous situation which, if not avoided, could result in property damage.
Do not use the 3M Molecular Detection Assay 2 - Campylobacter in the diagnosis of conditions in humans or animals.
The user must train its personnel in current proper testing techniques: for example, Good Laboratory Practices
ISO/IEC 17025 , or ISO 7218
(4)
To reduce the risks associated with a false-negative result leading to the release of contaminated product:
• Follow the protocol and perform the tests exactly as stated in the product instructions.
• Store the 3M Molecular Detection Assay 2 - Campylobacter as indicated on the package and in the product
instructions.
• Always use the 3M Molecular Detection Assay 2 - Campylobacter by the expiration date.
• Prepare 3M™ Campylobacter Enrichment Broth following product instructions
• Do not autoclave 3M Campylobacter Enrichment Broth
• Use the 3M Molecular Detection Assay 2 - Campylobacter with food and environmental samples that have been
validated internally or by a third party.
• Use the 3M Molecular Detection Assay 2 - Campylobacter only with surfaces, sanitizers, protocols and bacterial strains
that have been validated internally or by a third party.
• For an environmental sample containing Neutralizing Buffer with aryl sulfonate complex, perform a 1:2 dilution before
testing (1 part sample into 1 part sterile enrichment broth). Another option is to transfer 10 μL of the neutralizing buffer
enrichment into the 3M Lysis Solution tubes. 3M™ Sample Handling Products which include Neutralizing Buffer with
aryl sulfonate complex: BPPFV10NB, RS96010NB, RS9604NB, SSL10NB, SSL10NB2G, HS10NB, HS10NB2G, and
HS2410NB2G.
To reduce the risks associated with exposure to chemicals and biohazards:
• Perform pathogen testing in a properly equipped laboratory under the control of trained personnel. Incubated
enrichment media and equipment or surfaces that have come into contact with incubated enrichment media may
contain pathogens at levels sufficient to cause risk to human health.
• Always follow standard laboratory safety practices, including wearing appropriate protective apparel and eye
protection while handling reagents and contaminated samples.
• Avoid contact with the contents of the enrichment media and reagent tubes after amplification.
• Dispose of enriched samples according to current local/regional/national/ regulatory standards.
• Samples that have not been properly heat treated during the assay lysis step may be considered a potential biohazard
and should NOT be inserted into the 3M Molecular Detection Instrument.
To reduce the risks associated with cross-contamination while preparing the assay:
• Always wear gloves (to protect the user and prevent introduction of nucleases).
To reduce the risks associated with exposure to hot liquids:
• Do not exceed the recommended temperature setting on heater.
• Do not exceed the recommended heating time.
• Use an appropriate, calibrated thermometer to verify the 3M™ Molecular Detection Heat Block Insert temperature
(e.g., a partial immersion thermometer or digital thermocouple thermometer, not a total immersion thermometer). The
thermometer must be placed in the designated location in the 3M Molecular Detection Heat Block Insert.
To reduce the risks associated with cross-contamination while preparing the assay:
• Change gloves prior to reagent pellet hydration.
• Use of sterile, aerosol barrier (filtered), molecular biology grade pipette tips is recommended.
• Use a new pipette tip for each sample transfer.
• Use Good Laboratory Practices to transfer the sample from the enrichment to the lysis tube. To avoid pipettor
contamination, the user may choose to add an intermediate transfer step. For example, the user can transfer each
enriched sample into a sterile tube.
• Use a molecular biology workstation containing germicidal lamp where available.
• Periodically decontaminate laboratory benches and equipment (pipettes, cap/decap tools, etc.) with a 1-5% (v:v in
water) household bleach solution or DNA removal solution.
WWARNING
.
(5)
NOTICE
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EN (English)
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