3M MDA2CAM96 Mode D'emploi page 7

Table 3. Enrichment Protocols According to AOAC PTM
Sample Matrix
Whole carcass rinsed in
400 mL of BPW
(a) (b)
Bird part (1.8 to 2 Kg)
rinsed in 400 mL of
BPW
(a) (b)
Turkey carcass
sponge
(a) (b)
Raw ground poultry
(325 ± 32.5 g) rinsed in
1625 ± 32.5 mL BPW
(b)
Chicken nuggets
(a) If birds are treated with Cetylpyridinium chloride (CPC), it is necessary to add 5 mL per L of (Polysorbate 80; IUPAC:
Polyoxyethylene (20) sorbitan monooleate CAS 9005-65-6) to the prepared 3M Campylobacter Enrichment Broth.
Polysorbate 80 can be added to water before sterilizing or it can be added to sterile water before preparing 3M
Campylobacter Enrichment Broth.
(b) Alternatively, this matrix can be enriched with 30 mL of 2X blood-free Bolton Enrichment Broth (BF-BEB) for 48 ± 2 h
at 42 ± 1.0°C in microaerobic conditions. Transfer 20 µL of sample to 3M Lysis Solution.
(c) 3M Campylobacter Enrichment Broth should be used within 24 h of preparation. Medium should be at ambient
temperature (25-30°C) before use.
(d) Before collecting enrichment sample for analysis, gently massage the bottom of bag. After collecting the sample,
roll down the bag to prevent exposure of the enrichment to air. Additional sample may be required for re-testing or
confirmatory steps.
Preparation of the 3M™ Molecular Detection Speed Loader Tray
1. Wet a cloth or disposable towel with a 1-5% (v:v in water) household bleach solution and wipe the 3M Molecular
Detection Speed Loader Tray.
2. Rinse the 3M Molecular Detection Speed Loader Tray with water.
3. Use a disposable towel to wipe the 3M Molecular Detection Speed Loader Tray dry.
4. Ensure the 3M Molecular Detection Speed Loader Tray is dry before use.
Preparation of the 3M™ Molecular Detection Chill Block Insert
Place the 3M Molecular Detection Chill Block Insert directly on the laboratory bench: The 3M Molecular Detection Chill
Block Tray is not used. Use the block at ambient laboratory temperature (20 - 25°C).
Preparation of the 3M™ Molecular Detection Heat Block Insert
Place the 3M Molecular Detection Heat Block Insert in a dry double block heater unit. Turn on the dry block heater unit and
set the temperature to allow the 3M Molecular Detection Heat Block Insert to reach and maintain a temperature of 100 ± 1°C.
NOTE: Depending on the heater unit, allow approximately 30 minutes for the 3M Molecular Detection Heat Block Insert
to reach temperature. Using an appropriate, calibrated thermometer (e.g., a partial immersion thermometer, digital
thermocouple thermometer, not a total immersion thermometer) placed in the designated location, verify that the 3M
Molecular Detection Heat Block Insert is at 100 ± 1°C.
Preparation of the 3M™ Molecular Detection Instrument
1. Launch the 3M™ Molecular Detection Software and log in. Contact your 3M Food Safety representative to ensure you
have the most updated version of the software.
2. Turn on the 3M Molecular Detection Instrument.
3. Create or edit a run with data for each sample. Refer to the 3M Molecular Detection System User Manual for details.
Sample Size
30 mL of rinsate in BPW
30 mL of rinsate in BPW
1 sponge pre-moistened with
up to 25 mL of BPW
30 mL of product
mixture in BPW
25 g
Certificate #111803.
SM
3M Enrichment
Campylobacter Temperature
Enrichment (± 1°C)
Broth (mL)
(c)
30
30
25
30
225
6
EN (English)
Enrichment
Time
(hours) Volume (μL)
41.5 22-26
41.5 22-26
41.5 24-26
41.5 24-28
41.5 24-28
Sample
Analysis
(d)
20
20
20
20
20