3M MDA2CAM96 Mode D'emploi page 5

Instructions for Use
Follow all instructions carefully. Failure to do so may lead to inaccurate results.
Periodically decontaminate laboratory benches and equipment (pipettes, cap/decap tools, etc.) with a 1-5% (v:v in water)
household bleach solution or DNA removal solution.
The user should complete the 3M Molecular Detection System operator qualification (OQ) training, as described in the
"Installation Qualification (IQ) / Operational Qualification (OQ) Protocols and Instructions for 3M Molecular Detection
System" document .
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Media Preparation
Prepare 3M™ Campylobacter Enrichment Broth (CE250) following product instructions. Do not autoclave medium before
use. Use the prepared medium within 24 hours of preparation. Store prepared broth at 2-8°C
not be immediately used after preparation. Ensure media is tempered to 20-30°C before use.
Sample Collection
3M Campylobacter Enrichment Broth should not be used for bird rinsing or transport media. Collect and transport
samples following your established sample collection procedures.
Sample Enrichment
Table 2 present guidance for general enrichment protocols for food and environmental samples.
It is the user's responsibility to validate alternate sampling protocols or dilution ratios to ensure this test method meets the
user's criteria.
Sample Preparation
a. Carcass rinses and raw poultry part rinses
1. Rinse one eviscerated raw poultry carcass with 400 mL of buffered peptone water (BPW) for one minute. If rinsing
raw poultry parts, rinse 1.8 to 2 Kg (4 lb ± 10%) of bird parts with 400 mL of BPW
2. For carcass and raw poultry parts, allow excess liquid to drip before rinsing the sample to avoid transferring excess
processing liquid in the sample bag
3. For poultry that have been treated with Cetylpyridinium chloride (CPC), it is necessary to add 5 mL per L of
Polysorbate 80 (IUPAC: Polyoxyethylene (20) sorbitan monooleate; CAS 9005-65-6) to the prepared 3M
Campylobacter Enrichment Broth. Polysorbate 80 can be added to water before sterilizing to facilitate dissolution
or it can be added directly to sterile water before preparing 3M Campylobacter Enrichment Broth.
4. Aseptically transfer 30 mL of rinse to a sterile bag and add 30 mL of 3M Campylobacter Enrichment Broth.
b. Carcass sponge
1. Sponges should be hydrated before taking the sample with up to 25 mL of BPW before taking the sample
transporting the samples ensure that the bag is roll down and kept at 2-8°C.
2. Swab poultry carcass or collect sample with sponge.
3. Place swab into a sterile bag and add 25 mL of 3M Campylobacter Enrichment Broth. Ensure that the swab or
sponge is covered by the enrichment media.
c. Raw poultry products
1. Aseptically weight 325 ± 32.5 g of sample and place into a sterile bag. Add 1625 ± 32.5 mL of BPW to raw poultry
product. To disperse clumps, mix thoroughly by brief hand massaging.
2. After mixing, add 30 mL of the raw poultry product mixture to a sterile bag and then add 30 mL of 3M
Campylobacter Enrichment Broth and mix thoroughly.
d. Raw and ready-to-eat meat
1. Aseptically weight 25 g of sample and place into a sterile bag. A filter bag is recommended to facilitate sampling.
2. Add 225 mL of 3M Campylobacter Enrichment Broth.
3. Massage by hand to break up clumps, avoid creating bubbles when mixing. Do not process the bag by stomaching
or blending.
e. Primary production boot swabs
1. Collect sample with boot swabs or socks following your established sampling collection procedures.
2. Place ONE sock into a sterile bag and add 100 mL of 3M Campylobacter Enrichment Broth.
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