3M MDA2CAM96 Mode D'emploi page 8

NOTE: The 3M Molecular Detection Instrument must reach Ready state before inserting the 3M Molecular Detection
Speed Loader Tray with reaction tubes. This heating step takes approximately 20 minutes and is indicated by an
ORANGE light on the instrument's status bar. When the instrument is ready to start a run, the status bar will turn GREEN.
Lysis
Remove the bottom of 3M Lysis Solution Rack with a screwdriver before placing in in the 3M Molecular Detection Heat
Block Insert.
1. Allow the 3M Lysis Solution tubes to warm up by setting the rack at ambient temperature (20-25°C) overnight
(16-18 hours). Alternatives to equilibrate the 3M Lysis Solution tubes to ambient temperature are to set the 3M
Lysis Solution tubes on the laboratory bench for at least 2 hours, incubate the 3M Lysis Solution tubes in a 37 ± 1°C
incubator for 1 hour or place them in a dry double block heater for 30 seconds at 100°C.
2. Invert the capped tubes to mix. Proceed to next step within 4 hours after inverting.
3. Remove the enriched sample from the incubator.
3.1.1 Gently massage the bottom of the enrichment bag before transferring the sample to the 3M Lysis Solution tube.
3.1.2 Additional sample may be required for re-testing or confirmatory steps. After collecting the sample,
roll down the bag to minimize headspace and prevent exposure of enrichment to air. If confirmation of
presumptive results is required, proceed with confirmatory steps as soon as presumptive result is obtained.
4. One 3M Lysis Solution tube is required for each sample and the NC sample (sterile enrichment medium).
4.1 3M Lysis Solution tube strips can be cut to desired tube number. Select the number of tubes or 8-tube strips
needed. Place the 3M Lysis Solution tubes in an empty rack.
4.2 To avoid cross-contamination, decap one 3M Lysis Solution tube strip at a time and use a new pipette tip for each
transfer step.
4.3 Transfer enriched sample to 3M Lysis Solution tubes as described below:
Transfer each enriched sample into an individual 3M Lysis Solution tube first. Transfer the NC last.
4.4 Use the 3M™ Molecular Detection Cap/Decap Tool-Lysis to decap one 3M Lysis Solution tube strip - one strip at a
time.
4.5 Discard the 3M Lysis Solution tube cap - If lysate will be retained for retest, place the caps into a clean container
for re-application after lysis.
4.5.1 For processing of retained lysate, see Appendix A.
4.6 Transfer 20 µL of sample into a 3M Lysis Solution tube.
5. Repeat steps 4.4 to 4.6 as needed, for the number of samples to be tested.
6. When all samples have been transferred, transfer 20 µL of NC (sterile enrichment medium e.g. BPW) into 3M Lysis
Solution tube. Do not use water as a NC.
7. Verify that the temperature of the 3M Molecular Detection Heat Block Insert is at 100 ± 1°C.
8. Place the uncovered rack of 3M Lysis Solution tubes in the 3M Molecular Detection Heat Block Insert and heat for
15 ± 1 minutes. During heating, the 3M Lysis Solution will change from pink (cool) to yellow (hot).
8.1 Samples that have not been properly heat treated during the assay lysis step may be considered a potential
biohazard and should NOT be inserted into the 3M Molecular Detection Instrument.
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EN (English)
20 µl