3M MDA2CAM96 Mode D'emploi page 6

f. Drag swab
1. Collect sample with pre-moistened swab device following your established sampling collection procedures.
2. Place swab into a sterile bag and add 100 mL of 3M Campylobacter Enrichment Broth.
Incubation of Enrichment
1. Roll down the bag to minimize headspace and prevent exposure of enrichment to air. Gently massage the bag for about
10 ± 2 seconds. Do not process the bag by stomaching or blending and avoid creating bubbles when mixing.
2. Incubate the bag aerobically at 41.5 ± 1°C, refer to table 2 for the appropriate incubation time.
WARNING: Should you select to use neutralizing buffer that contains aryl sulfonate complex as the hydrating solution
for the sponge, it is required to perform a 1:2 dilution (1 part sample into 1 part sterile enrichment broth) of the enriched
environmental sample before testing in order to reduce the risks associated with a false-negative result leading to the
release of contaminated product. Another option is to transfer 10 µL of the Neutralizing Buffer enrichment into the 3M
Lysis Solution tubes.
It is the user's responsibility to validate alternate sampling protocols or dilution ratios to ensure this test method meets the
user's criteria.
Table 2. General Enrichment Protocols.
Sample Matrix
• Carcass rinses
(a)
• Bird part rinses
(a)
• Carcass sponge
(a)
• Raw meat
• Ready-to-eat meat
• Boots swabs from
primary production
• Drag swab from
primary production
(a) If birds are treated with Cetylpyridinium chloride (CPC), it is necessary to add 5 mL per L of (Polysorbate 80; IUPAC:
Polyoxyethylene (20) sorbitan monooleate CAS 9005-65-6) to the prepared 3M Campylobacter Enrichment Broth.
Polysorbate 80 can be added to water before sterilizing or it can be added to sterile water before preparing 3M
Campylobacter Enrichment Broth.
(b) 3M Campylobacter Enrichment Broth should be used within 24 h of preparation. Medium should be at ambient
temperature (25-30°C) before use.
(c) Before collecting enrichment sample for analysis, gently massage the bottom of bag. After collecting the sample,
roll down the bag to prevent exposure of the enrichment to air. Additional sample may be required for re-testing or
confirmatory steps.
Specific Instructions for Validated Methods
AOAC® Performance Tested
In AOAC Research Institute PTM
effective method for the detection of Campylobacter. The matrices tested in the study are shown in Table 3.
Sample Size
30 mL of rinsate in BPW
1 sponge pre-moistened with
up to 25 mL of BPW
25 g
1 boot swab
1 pre-moistened device
(PTM) Certificate #111803
SM
studies, the 3M Molecular Detection Assay 2 - Campylobacter was found to be an
SM
3M Enrichment
Campylobacter Temperature
Enrichment (± 1°C)
Broth (mL)
(b)
30 41.5
25 41.5
225 41.5
100 41.5
100 41.5
5
EN (English)
Enrichment Sample
Time Analysis
(hours) Volume (μL)
22-26 20
22-26 20
24-28 20
22-26 20
22-26 20
(c)