Agilent InfinityLab Poroshell 120 HILIC Guide D'utilisation page 7

Working With Normal-Phase Columns
Every normal-phase column is tested before shipment and shipped in a
normal-phase test eluent, with the exception of ZORBAX Eclipse XDB-CN,
InfinityLab Poroshell 120 EC-CN, InfinityLab Poroshell 120 HILIC, InfinityLab
Poroshell HILIC-OH5, InfinityLab Poroshell HILIC-Z, and ZORBAX HILIC Plus
columns which are shipped in reversed-phase solvents. Care should be
taken to make sure the column has been properly equilibrated prior to use.
This will ensure reproducibility from analysis to analysis and help prevent
retention time drifting.
Normal-Phase Column Operating Parameters:
pH and Temperature
Phase
InfinityLab Poroshell 120 EC-CN*
ZORBAX NH2
InfinityLab Poroshell 120 HILIC*
ZORBAX Rx-SIL
ZORBAX SIL
ZORBAX HILIC Plus*
ZORBAX CN
ZORBAX Eclipse XDB-CN*
Polaris NH2
Polaris Si-A
Pursuit XRs Si
* Shipped in reversed-phase solvents.
See the Working with HILIC section for more information.
†
Operating at extreme ends of pH and temperature ranges will have a
significant impact on column lifetime.
Recommended
pH range
pH 2.0 to 8.0
pH 2.0 to 7.5
pH 1.0 to 8.0
†
†
pH 2.0 to 8.0
pH 2.0 to 9.0
pH 2.0 to 8.0
pH 1.5 to 10.0
pH 2.0 to 8.0
ENGLISH
Maximum Suggested
operating operating
temperature temperature
60 °C 30 °C
60 °C 30 °C
Limited
only by the
temperature
limits of the
mobile phase
60 °C 30 °C
60 °C 30 °C
60 °C 30 °C
60 °C 30 °C
60 °C 30 °C