Agilent InfinityLab Poroshell 120 HILIC Guide D'utilisation page 6

Tips for Getting the Best Chromatographic Results
• Optimize your instrument by minimizing tubing lengths between
components to reduce extra column volume and band broadening. Use
0.12 mm id red tubing for normal operations. For narrow bore (2.1 mm
or less) high resolution columns, such as InfinityLab Poroshell 120 or
ZORBAX RRHD, use 0.075 mm id black tubing on UHPLC systems. Learn
about capillary options at agilent.com/chem/lccapillaries.
• When using normal-phase columns, ensure that your LC instrument's
rotor seals, pump seals, tubing, and all other components are compatible
with normal-phase solvents.
• Make sure your data collection rate is optimized for your column. Use
a higher collection rate for Fast LC columns (InfinityLab Poroshell 120,
RRHT, and RRHD).
• Use Agilent certified lamps in your LC instruments for best performance.
• Use sample filtration or other sample prep as appropriate for your
sample. Learn more at agilent.com/chem/sampleprep.