Agilent InfinityLab Poroshell 120 HILIC Guide D'utilisation page 5

Column Usage Tips
• Modern columns are robust and are designed to operate for long
periods under normal chromatographic conditions. You can maximize
column lifetime by running it within specifications. Always review the
specifications before putting in place a final method.
• While generally not harmful to the column, reverse flow should be
avoided.
• Always use high purity reagents and chromatography grade solvents or
better to prepare your mobile phase.
• Disassembling a column will degrade column performance.
• New columns contain a mixture of organic solvents. See your QC
Performance Report for the solvent composition in your column. Initially,
care should be taken not to pass any mobile phase through the column
that may cause a precipitate or an emulsion to form.
• Agilent normal-phase columns are compatible with water and all
common organic solvents.
• The use of a guard column is recommended to protect your column and
increase its lifetime.
• Columns should not be maintained at high pH or elevated temperature
when not in use.
• Avoid use of this column outside recommended pH range for column
phase (see next page). Expect reduced lifetime when operating outside
the recommended pH and temperature ranges.
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