Leica DM5500 B Manuel D'utilisation page 50

7. Startup
• Select an objective with moderate magnifica-
tion (10x-20x).
• If necessary, activate the transmitted light
axis by pushing the TL/IL button (43.1).
• Choose "bright field" as the contrast method
by pressing the BF key (one of the variable
function keys at the stand, STP6000 or at the
Leica SmartTouch).
• Insert the specimen in the stage's specimen
holder (45.1).
• Focus on the specimen with the SmartMove,
STP6000 or focus wheel.
• Set the light intensity using the INT keys
(43.3).
• Close the field diaphragm with the FD function
key (43.4) until the edge of the diaphragm ap-
pears in the specimen plane.
Fig. 46 Condenser centering
1 Centering bolts
1
50
• Using the condenser height adjuster (45.2),
adjust the condenser until the edge of the
field diaphragm appears in sharp relief.
• If the image does not appear in the middle of
the field of view (47c), the condenser must be
moved into the middle of the field of view with
the help of the two centering bolts (46.1).
• Open the field diaphragm just enough for it to
disappear from the field of view (47d).
The light of the condenser depends on the thick-
ness of the specimen. It has to be adjusted for
each specimen.
Fig. 47
a Field diaphragm not focused, not centered
b Field diaphragm focused, but not centered
c Field diaphragm
Diameter is too small, however
d Field diameter (light) = Field diameter (view)
(Köhler Illumination)
1
Caution:
Köhler Illumination
a
c
b
d