Materials Required; Quality Control - HemoCue Hb 201+ Manuel D'utilisation

In case of peripheral circulatory failure, hemoglobin measure-
ments from capillary sampling may be misleading. Confirmation
of an unacceptable or unexpected laboratory result is considered
as a common, well established routine in the laboratory. Extra cel-
lular fluid present in capillary samples due to individual physio-
logy or the affects of unacceptable capillary sampling technique
can cause false hemoglobin results.
One of several pre analytical factors associated with technique
revolves around the size and action mode of the lancet and how it
is used. This and other factors associated with capillary sampling
technique can have an impact on the puncture, blood flow and
ultimately on the results obtained. Additional factors unrelated to
sampling technique, such as an individual's capillary circulation
status, must also be recognized as potential causes of false results.
Therefore when testing for hemoglobin using capillary blood as
the sample, confirmation of an unacceptable or unexpected result
is needed to rule out potential pre analytical factors as the cause.
This is particularly helpful when a cut off value is used, i.e. when
screening for nutritional status based on hemoglobin. Since the
individual performing the test may not always be aware of all the
potential pre analytical factors or even those that are directly or
indirectly associated with sampling techniques, a second finger
stick should be performed when wanting to rule out pre analyti-
cal factors as a cause of an unacceptable or unexpected result.
Performance of the second finger stick by a different individual
may aid in lessening the affect of the factors that could have been
technique related.
Always handle blood specimens with care, as they might be
infectious. Consult local environmental authorities for proper
disposal. Always wear protective gloves when handling blood
specimens. The microcuvette is for single use only.

Materials required

• HemoCue Hb 201 + Analyzer
• HemoCue Hb 201 Microcuvettes
• Lancet (for capillary samples)
• Pipette or other transfer device (for venous, arterial or control
material samples)
• Lint-free wipe (non-fraying)
• Hydrophobic surface (for venous, arterial or control material
samples)

Quality Control

The HemoCue Hb 201 + Analyzer has an internal quality con-
trol, the "self test". Every time the analyzer is turned on, it will
automatically verify the performance of the optronic unit of the
anayzer. This test is performed every second hour if the analyzer
remains switched on.
Follow local guidelines regarding quality control procedures.
If a quality control test is required by local or other regulations
and therefore should be performed, only use controls recommen-
ded by HemoCue, see relevant package insert for more informa-
tion.
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Expected values
Adult Males 130–170 g/L (13.0–17.0 g/dL, 8.1–10.5 mmol/L)
Adult Females 120–150 g/L (12.0–15.0 g/dL, 7.4–9.3 mmol/L)
Infants, after neonatal period 110–140 g/L (11.0–14.0 g/dL,
6.8–8.7 mmol/L)
Children, two years to teenage: gradual increase to adult normals.
Due to a wide range of conditions (dietary, geographical,
etc) which affect normal values, it is recommended that each
laboratory establish its own normal range.
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