Materials Required; Quality Control; Measuring Range; Limitations Of The Procedure - HemoCue Manuel D'utilisation

Materials required

• HemoCue Plasma/Low Hb Photometer
• HemoCue Plasma/Low Hb Microcuvettes
• Pipette or other transfer device
• Disposable pipette tips
• Lint-free wipe (non-fraying)
• Hydrophobic surface

Quality Control

Two levels of liquid control is recommended to be run on the day of
use, or follow local guidelines regarding quaIity controI procedures.
Only use controIs recommended by HemoCue, see relevant package
insert for more information.

Measuring range

The system is linear from between 0.3–30.0 g/L (0.03–3.00 g/dL,
30–3,000 mg/dL, 0.02–1.90 mmol/L). Caution should be taken when
evaluating instrument readings between 0 and 0.3 g/L (0–0.03 g/dL,
0–30 mg/dL, 0–0.02 mmol/L).
Results
The measured hemoglobin value is read directly from the HemoCue
Plasma/Low Hb Photometer. No calculations are necessary.
Applications sheets for use in determining blood loss and for
calculating the amount of free hemoglobin in salvaged or stored
blood are available from HemoCue AB.

Limitations of the procedure

a) If 'HHH' is displayed, the result exceeds the measuring range of
the system.
b) For values above 30.0 g/L (3.00 g/dL, 3,000 mg/dL, 1.90 mmol/L)
use a suitable laboratory method or the HemoCue Hb 201 system.
c) Sulfhemoglobin is not measured with this method.
d) Levels of bilirubin up to 340 µmol/L (20 mg/dL) do not influence
the assay.
e) The presence of lipid may interfere with the hemoglobin
determination. Therefore, samples which are visibly turbid should
be filtered (pore size 0.2 µm). If the user feels uncertain about the
degree of turbidity, we recommend filtering the sample.
f) The performance characteristics of this system have not been
determined using samples obtained from uremic patients.
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