Calibration - Hach Thermo Fisher TenSette plus Manuel De L'utilisateur

Seq + Aspirate
The sequential aspirate mode enables serial aspirating of different volumes.
1. Select Program > Edit, then select with the scroll key Seq + Aspirate and accept with OK.
2. First choose the number of volumes (maximum 20) with the scroll key and accept with OK. VOL 1
is displayed and the highest possible volume is flashing.
3. Select the first volume with the scroll key and accept with OK. Now VOL 2 is displayed and the
highest possible remaining volume is flashing.
4. Select the second volume with the scroll key and accept with OK. After selecting the last volume,
the total volume is shown on display and speed-in is flashing for selecting the pipettings speeds.
After selecting the speeds, the first volume is shown on the display and the pipette is ready for
pipetting.
5. Fill a clean reagent reservoir with the liquid to be dispensed.
6. Dip the tip under the surface of the liquid and press the trigger. This action will take up first
volume and the next volume is displayed.
7. Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess
liquid.
8. Dip the tip under the surface of the next liquid and press the trigger. This action will take up the
current volume and the next volume appears on the display.
9. Repeat steps 2 and 3 until last volume is drawn into the tip. The total volume is shown on the
display.
10. Deliver the total volume by pressing the trigger and hold it down to empty the tip.
11. Release the trigger to return to the ready position.
12. Change the tip and continue pipetting.
Note: The pipette can be emptied at any time by pressing Cancel left selection key. Refer to
on page 7.

Calibration

All Hach TenSette plus are factory calibrated and adjusted to give the volumes as specified with
distilled or deionized water. Normally, the pipettes do not need adjustment, but they are constructed
to permit recalibration and adjustment for liquids of different temperature and viscosity.
Device requirements and test conditions
An analytical balance must be used. The scale graduation value of the balance should be chosen
according to the selected test volume of the pipette. Refer to
If the uncertainty of measurement of the balance is known, this may be used instead of the
repeatability and linearity.
Test liquid: Water, distilled or deionized, "grade 3" water conforming ISO 3696. Tests are done in a
draft-free room at a constant (±0.5 °C/ ±33 °F) temperature of water, pipette and air between 15 °C
(59 °F) to 30 °C (86 °F). The relative humidity must be above 50%.
Volume range Readable graduation Precision repeatability(s) and
10 to 100 µL 0.01 mg
> 100 µL 0.1 mg
12 English
Table 1 Test conditions
linearity
0.02 mg
0.2 mg
Table 1.
Uncertainty of measurement
0.02 µL
0.2 µL
Figure 2