Operating The Certomat® Ctplus; Safety Instructions - Sartorius Stedim Biotech CERTOMAT CTplus Mode D'emploi Original

3 Operating the CERTOMAT® CTplus
3.1

Safety instructions

Dangers can arise from the media used in the
CERTOMAT® CTplus that are specific to the media and
the process. This applies to shaker cultures with living
cells and viruses as well as aggressive or inflammable
media, for example. This manual cannot, however,
describe specific dangers and the resulting safety
measures in more detail.
The operating company must instruct its personnel of
the dangers associated with handling the media or
contaminated devices and ensure adherence to the
safety regulations.
The following safety measures must be observed:
When using potentially hazardous media, only
authorised personnel should be allowed to remain at the
workplace and to operate the cabinet shaker;
unauthorised personnel are not allowed to manipulate
the device.
Operators should wear appropriate work clothes and
personal protection gear such as gloves, safety goggles
and respiratory protection if necessary.
You must only use equipment configurations that are
approved by Sartorius Stedim Biotech GmbH for use
with the CERTOMAT® CTplus. Check that all parts are in
a perfect condition, especially the glass vessels. Never
use damaged (glass) parts.
Never operate the shaker drive without a tray. Always
switch the shaker drive off before inserting or taking
out containers to be shaken or removing or inserting
the tray.
Take care when inserting the tray as well as the
assemblies, vessel holder systems and accessory parts.
Centre the tray and distribute the load evenly on the
shaker table. Vessels must not become loose, being
thrown out or strike one another.
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Reduce the shaker speed if the cabinet shaker vibrates
strongly (due to resonant effects), impedes other devices
in the vicinity or if the cabinet assembly becomes
unstable during the operation of stacked cabinets.
Critical operating conditions are also possible during
normal operation. Certain shaker speeds can induce
vibrations in the cabinet shaker (resonance effects)
depending on the load. The drive includes a
counterweight that is not capable of fully compensating
such vibrations. Transfer of the vibrations to the
installation area is then possible and can impede other
devices in the vicinity.
If you incubate media in open vessels, you should work
with the lowest possible shaker speeds. If media are
spilled, e.g. following breakage of glass vessels, you
should clean the shaker cabinet immediately and
remove the broken glass or any foreign bodies. Make
sure that foreign bodies cannot get between the shaker
table and the drip pan or into the shaker drive. See also
Chapter 11.3 Measures in the event of malfunction
(troubleshooting)
CO as the control gas.
2
Check the type of gas and its suitability for the
designated purpose. Make sure that all lines are
connected correctly and cannot become loose from the
connection. Check whether the gas pressure is adjusted
to the specified value.
If gas lines are not connected properly and the wrong
gas pressure is used, CO can escape. Elevated CO
2
are hazardous to health and can lead to suffocation.
Ventilate the rooms in which CO
gas from time to time. Gas content increases in closed
rooms and can reach concentrations that are hazardous
to health. Avoid direct breathing in of the air from the
interior if CO is used.
2
values
2
is used as the control
2