HemoCue Hb 201+ Manuel D'utilisation page 26

Specifications
US
General
The HemoCue Hb 201
of the total amount of hemoglobin in whole blood. The system
consists of a specially designed analyzer with specially designed
microcuvettes containing dried reagents. The microcuvette serves
as pipette, reaction vessel and as a measuring microcuvette.
No dilution is required. The hemoglobin measurement takes place
in the analyzer, which follows the progress of the reaction until
the steady state has been reached. The system is factory calibrated
against the hemoglobincyanide (HiCN) method, the international ref-
erence method for the determination of the hemoglobin
concentration in blood.
Intended Use
Quantitative determination of hemoglobin in capillary, venous or
arterial blood. The HemoCue Hb 201
determination of hemoglobin in whole blood using a specially
designed analyzer, the HemoCue Hb 201
designed microcuvettes, the HemoCue Hb 201 Microcuvettes.
HemoCue Hb 201 Microcuvettes are for In Vitro Diagnostic use
only. The HemoCue Hb 201
HemoCue Hb 201 Microcuvettes.
Theory
The reaction in the microcuvette is a modified azidemethemoglobin
reaction. The erythrocyte membranes are disintegrated by sodium
deoxycholate, releasing the hemoglobin. Sodium nitrite converts
the hemoglobin iron from the ferrous to the ferric state to form
methemoglobin, which then combines with azide to form
azidemethemoglobin.
+ is a system used for the determination
+ system is used for quantitative
+
analyzer, and specially
+ analyzer is only to be used with
Reagents
40 % w/w Sodium Deoxycholate, 18 % w/w Sodium Azide,
20 % w/w Sodium Nitrite, 22 % w/w nonreactive ingredients.
Sample material
Capillary, venous or arterial blood may be used. Appropriate anti-
coagulants in solid form (eg EDTA, heparin or heparin/fluoride)
may be used. Mix all samples thoroughly on a mechanical mixer
for at least 2 minutes or invert the tube 8-10 times by hand.
Alternatively, follow the local recommendations. Hemoglobin
remains unchanged for days, provided that the blood does not
become infected. If the specimen has been stored in a refrigerator,
it will be viscid and the blood should be allowed to warm up to
room temperature before mixing.
Storage and environmental requirements of the
HemoCue Hb 201 Microcuvettes
Use the HemoCue Hb 201 Microcuvettes prior to their
expiration date. The expiration date is printed on each package.
Storage of microcuvettes kept in a vial
The microcuvettes are to be stored at room temperature (59 – 86 °F,
15– 30 °C). Do not refrigerate. The expiration date of the Hemo-
Cue Hb Microcuvettes in a sealed vial is printed on the vial.
Once the seal is broken, the microcuvettes are stable for three
months. Always keep the container properly closed.
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