Differential Interference Contrast Dic Observation (Optional); Koehler Dic Transmitted Light - Optika Italy B-1000 Serie Manuel D'utilisation

14. Differential Interference Contrast DIC Observation (optional)
• The microscope allows the observation in Differential Interferential Contrast (DIC) with two different methods:
Koehler DIC and Nomarski DIC.
• Samples with rapid decay must first be observed in Fluorescence and then in DIC.
• The Koehler DIC method is the simplest both from the point of view of installation and from the point of view
of use, while the Nomarski DIC method provides for a more complex setup.
• Both methods work in transmitted light but can be used in combination with fluorescence observation re-
flected light and therefore the transmitted light only set-up is different from the combined fluorescence obser-
vation set-up.

14.1 Koehler DIC transmitted light

The observation in Koehler DIC in transmitted light
requires the kit consisting of the following accesso-
ries: Polarizer ①, Analyzer for transmitted light ②,
Interferential green filter IF550 ③ and DIC slider ④.
(Fig. 62)
1. Place the polarizer on the lens at the base of the
microscope.
2. Remove the dummy slider from the nosepiece
and insert the analyzer into the hole of the dum-
my slider, then insert the assembly ⑤ into the
slot ⑥. (Fig. 63)
3. Remove the slide from the stage.
4. Rotate the polarizer at the base of the microscope
to achieve maximum darkening of the eyepieces.
5. Once the maximum darkening is achieved, remo-
ve the analyzer from the dummy slider and insert
it into the DIC prism. Now insert the DIC slider ⑦
into the slot ⑥. (Fig. 64)
6. Close the condenser aperture diaphragm a little.
7. Put a specimen on the stage and focus.
8. Begin the observation by rotating the DIC ⑧ sli-
der knob to obtain a three-dimensional sample
effect. (Fig. 65)
• For a better effect on the image you can use the
green filter IF550 which must be placed on top of
the polarizer.
③
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①
④
②
⑤
⑥
⑦
⑥
⑧
Fig. 62
Fig. 63
Fig. 64
Fig. 65