Checking Modulation Contrast Slit Diaphragms - Leica Microsystems DMI3000 B Mode D'emploi

7. Start-up
Leica DMI4000 B and Leica DMI6000 B:
• Press the BF (bright fi eld) button (one of the
variable function buttons on the stand).
• Select the objective with the lowest magnifi -
cation.
• Focus on the specimen.
• Select the objective with the lowest magnifi ca-
tion and eyepoint C.
Leica DMI3000 B:
• Select the light ring for your current objective
on the condenser.
Leica DMI4000 B and Leica DMI6000 B:
• Press the PH (phase contrast) button (one of
the variable function buttons behind the focus
wheels). The light ring will be selected in the
condenser.
• Slide the front slider with the phase rings to
position C (A and C refer to the eyepoint of the
objective. For the eyepoint of your objective,
please refer to the included objective list or the
engraving on the objective itself.)
• Instead of an eyepiece, place a focusing tel-
escope (Fig. 93) in the observation tube or acti-
vate the Bertrand lens (pull rod (94.1) on tube).
• Focus the ring structure (95a) by loosening
the clamping ring (93.2) somewhat and moving
the eyelens (93.1), or focus the Bertrand lens
(94.2).
• Retighten the clamping ring.
76
• If the light ring and the phase ring are not
shown as arranged in Fig. 95c, the light ring
must be centered.
• Insert the centering key in the opening provid-
ed on the front slider
• Turn the centering keys until the dark ring
(phase ring in the objective) is congruent with
the slightly narrower bright ring (light ring in
condenser) (95 c).
• Remove the centering keys after centering.
7.8 Checking modulation contrast slit dia-
phragms
If your microscope is prepared for integrated
modulation contrast, its condenser will be
equipped with slit diaphragms suitable for the
objectives.
The slit diaphragms have been centered at the
factory.
Their proper location should be checked, how-
ever.
Note:
Each objective has its own slit diaphragm as-
signed to it in the condenser disk. The test must
therefore be performed for each objective.
Open the cover at the top right side of the con-
denser. The various
numbered openings for the inserts are now vis-
ible. Ensure that all of the slit diaphragms are
fi rmly seated and that none of the retaining
screws are loose. If a part has loosened, please
see Chapter 6.5 Installation of Condensers.