Leica BIOSYSTEMS BOND Hybridization Solution AR9037 Mode D'emploi page 3

BOND Hybridization Solution
Catalog No: AR9037
Intended Use
This reagent is for in vitro diagnostic use.
BOND Hybridization Solution is intended to be used for the dilution of in situ hybridization (ISH) probes for use on the automated BOND
system (includes BOND-MAX system and BOND-III system).
The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and
should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Summary and Explanation
ISH allows visualization of specific nucleic acids in tissue sections (see "Using BOND Reagents" in your BOND user documentation).
The BOND system (includes BOND-MAX system and BOND-III system) provides an automated method of in situ hybridization
on formalin-fixed, paraffin-embedded tissue sections. BOND Hybridization Solution has been developed for the dilution of in situ
hybridization probes for use on the automated BOND system (includes BOND-MAX system and BOND-III system).
Reagents Provided
BOND Hybridization Solution.
Total volume = 100 mL.
Dilution and Mixing
BOND Hybridization Solution is ready to use. Reconstitution, mixing, dilution or titration of this reagent is not required.
Materials Required But Not Provided
Refer to "Using BOND Reagents" in your BOND user documentation for a complete list of materials required for specimen treatment and
ISH staining using the BOND system (includes BOND-MAX system and BOND-III system).
Storage and Stability
Store at 2–8 °C. The product is stable under these conditions up to the expiry date indicated on the container label. Do not use after the
expiration date indicated on the bottle label.
There are no obvious signs indicating contamination and/or instability. Appropriate positive and negative tissue controls should be run at
the same time as test tissue.
BOND Hybridization Solution contains a small quantity of sodium dodecyl sulfate (SDS). This can appear as a white precipitate when
the solution is first removed from 2–8 °C. The precipitate disappears when the solution reaches room temperature. The presence of the
precipitate does not affect the performance of the solution.
Return to 2–8 °C immediately after use.
Storage conditions other than those specified above must be verified by the user
Precautions
• This product is intended for in vitro diagnostic use.
BOND HYBRIDIZATION
SOLUTION
Contains Formamide (<50%)
and Dextran Sulphate
(<30%).
GHS07: Exclamation mark.
GHS08: Health hazard.
Signal words: Danger.
• To obtain a copy of the Safety Data Sheet contact your local distributor or regional office of Leica Biosystems, or alternatively, visit the
Leica Biosystems Web site, www.LeicaBiosystems.com
• Specimens, before and after fixation, and all materials exposed to them, should be handled as if capable of transmitting infection and
disposed of with proper precautions
reagents or specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Seek
medical advice.
• Consult Federal, State or local regulations for disposal of any potentially toxic components.
• Minimize microbial contamination of reagents or an increase in non-specific staining may occur.
• Retrieval, incubation times or temperatures other than those specified may give erroneous results. Any such change must be
validated by the user.
H360D:
May damage the unborn child.
H315: Causes skin irritation.
H319: Causes serious eye
irritation.
. Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with
2
.
1
P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read
and understood.
P260: Do not breathe dust/fumes/gas/mist/vapours/spray.
P308+313: If exposed or concerned: Get medical advice/attention.
P314: Get medical advice/attention if you feel unwell.
P264: Wash hands thoroughly after handling.
P280: Wear protective gloves/protective clothing/eye protection/
face protection.
P305+351+338: IF IN EYES: Rinse cautiously with water for
several minutes. Remove contact lenses, if present and easy to do.
Continue rinsing.
P337+313: If eye irritation persists: Get medical advice/attention.
P302+352: IF ON SKIN: Wash with plenty of soap and water.
P332+313: If skin irritation occurs: Get medical advice/attention.
P362+P364: Take off contaminated clothing and wash before
reuse.
AR9037
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