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Recommended starting gradients
Stationary Phase
Most reversed-phase
columns
ZORBAX Eclipse PAH*
ZORBAX Bonus‑RP
* Best column lifetime is achieved at <40 °C.
Usage Notes
5% methanol or acetonitrile initially and 100%
methanol or acetonitrile as the final solvent.
30 or 40% acetonitrile initially, to 100%
acetonitrile as final solvent. It may be necessary
to cool column to 15 to 20 °C for improved
resolution.
Lower concentration of organic mobile phase
modifier is required for compound elution,
compared to traditional long-chain alkyl
stationary phases.
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