Agilent AdvanceBio Série Guide D'utilisation page 6

Column operating parameters: pH and temperature
Phase
Poroshell 120 CS-C18
Poroshell HPH
Poroshell 120 SB-C18,
StableBond C18,
ZORBAX Rx-C8,
ZORBAX SB-Aq,
ZORBAX SB‑Phenyl,
SB-C3, Poroshell 300,
300SB-C3
ZORBAX Eclipse Plus C18
and C8, Eclipse XDB-C8,
XDB‑C18, XDB‑Phenyl,
Poroshell 120 Bonus-RP,
Poroshell 120 EC-C18 and
EC-C8, ZORBAX Bonus-RP,
HC-C18(2) and TC-C18(2)
ZORBAX Extend C18
Poroshell 120 PFP,
Poroshell 120 Phenyl-Hexyl,
ZORBAX ODS,
ZORBAX Phenyl,
ZORBAX Eclipse Plus
Phenyl‑Hexyl,
ZORBAX XDB-CN,
ZORBAX Eclipse PAH,
All Pursuit and Polaris
bonded phases
ZORBAX Eclipse PAH
ZORBAX TMS
InfinityLab PFAS delay
column
Note: All silica-based packings have some solubility in pH >6 aqueous mobile
phases. When using silica-based columns at pH >6, best column lifetime is
obtained at lower temperatures (40 °C max) using low buffer concentrations
in the range of 0.01 to 0.02 M. Operating at extreme ends of pH and
temperature ranges will have a significant impact on column lifetime.
Recommended
pH Range
pH 1.0 to 11.0
pH 2.0 to 11.0
pH 1.0 to 8.0
pH 2.0 to 9.0
pH 2.0 to 11.5
pH 2.0 to 8.0
pH 2.0 to 8.0
pH 2.0 to 7.0
pH 2.0 to 9.0
Maximum Operating
Temperature
90 °C
60 °C
90 °C (StableBond C18
and Poroshell 120 SB-C18)
80 °C (Rx-C8 and SB-Aq,
SB-C3 and SB-Phenyl)
70 °C for pH <5.0; 40 °C
for pH 5.0 to 8.0 (Poroshell
300 and 300SB-C3)
60 °C
60 °C (40 °C at high pH)
60 °C
60 °C at pH <6.0
60 °C
60 °C