Leica DM1000 Mode D'emploi page 53

Problem
Phase Contrast
No phase contrast is possible.
Polarization
No polarization contrast is possible.
Fluorescence
The image is completely dark (no fluorescence).
The fluorescence is too weak.
Cause/Remedy
The specimen is too thick, too thin or too
brightly stained.
Refractive index of mounting medium and
specimen is identical so that there is no phase
jump.
The cover glass is not placed evenly.
Check the right light ring (→ p. 44).
Check the centering of the light rings
(→ p. 29).
Check the condenser centering.
Open the aperture diaphragm completely.
Bring the polarizer and analyzer into cross po-
sition until they reach maximum darkness
(without specimen) (→ p. 45).
Open the shutter (→ p. 46).
Check the antigen-antibody combination.
Insert a new lamp (→ p. 21ff).
Center the lamp (→ p. 31ff).
Insert a new lamp (→ p. 21ff).
11. Trouble shooting
53