Agilent InfinityLab Poroshell 120 Guide D'utilisation page 8

Use of Chiral columns in Supercritical Fluid
Chromatography (SFC)
Agilent Poroshell 120 Chiral columns are suitable for SFC. Column pressure
and pH limits remain valid in SFC. Please keep in mind that pH in SFC is not
directly comparable with pH values of aqueous solutions. Therefore, the
apparent pH in CO /organic modifier might differ.
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Column equilibration
Before use, flush the column with 10–30 volumes of methanol or isopropyl
alcohol to completely flush out shipping solvents. Then, equilibrate the
column with approximately 10–20 column volumes of mobile phase at
starting conditions.
Column storage
Make sure that you completely remove additives such as acids, bases,
or buffers to prevent damage to the column. Flush the column with
10–20 column volumes of ethanol to remove all carbon dioxide. Do not
store columns in a mixture of CO and MeOH, as CO will evaporate and
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dry out the stationary phase. Properly seal with end plugs to prevent
solvent evaporation.