Agilent InfinityLab Poroshell 120 Guide D'utilisation page 6

Recommended Screening Mobile Phases
Column
Agilent InfinityLab
Poroshell Chiral-T
and Chiral-V
Agilent InfinityLab
Poroshell Chiral-CD
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Poroshell Chiral-CF
Recommended Method Optimizations
Column
Agilent InfinityLab
Poroshell Chiral-T
and Chiral-V
Agilent InfinityLab
Poroshell Chiral-CD
Agilent InfinityLab
Poroshell Chiral-CF
Screening mobile phases (isocratic)
Polar ionic: 100/0.2 wt% MeOH/ammonium formate
Polar organic: 60/40/0.3/0.2 ACN/MeOH/acetic acid/TEA
Reversed-phase: 30/70 MeOH/20 mM ammonium formate
(pH 4)
Normal-phase: 20/80/0.3/0.2 EtOH/heptane/acetic acid/TEA
Reversed-phase: 30/70 ACN/20 mM Ammonium formate
buffer (pH = 4)
Polar organic: 60/40/0.3/0.2 ACN/MeOH/acetic acid/TEA
Polar organic: 60/40/0.3/0.2 ACN/MeOH/acetic acid/TEA
Normal-phase: 20/80/0.3/0.2 EtOH/heptane/acetic acid/TEA
Recommended method optimization
Polar ionic mode: Change concentration of salts between
0.01 wt% and 1 wt%
Polar organic: Change ratio of ACN to MeOH
Reversed-phase: Change organic/buffer ratio
Change different organic solvents (ACN or MeOH)
Change pH for ionizable compounds (typically pH 3-5 for
bases and pH 5-7 for acids)
Normal-phase: Change ratio of alcohol to alkanes, change
type of alcohols
Reversed-phase: Change organic/buffer ratio
Change pH for ionizable compounds
Polar organic: Change ratio of ACN to MeOH
Polar organic: Change ratio of ACN to MeOH
Normal-phase: Change ratio of alcohol to alkanes, and/or
change type of alcohol