Troubleshooting; Dv20, D20; Dv50, D50; Dv100, D100 - HTL DISCOVERY Comfort Mode D'emploi

Range
of the pipette
Model
volumes
[μl]
DV2, D2 0.1 - 2
DV10, D10 0.5 - 10

DV20, D20

2 - 20

DV50, D50

5 - 50

DV100, D100

10 - 100

DV200, D200

20 - 200

DV250, D250

50 - 250

DV1000, D1000

100 - 1000

DV5000, D5000

500 - 5000

DV10000, D10000

1000 - 10000
• Perform 5 aspirations, weigh each one and calculate
the average value of the aspirations
• Calculate average aspirated volume in μl multiplying
the average aspiration amount [mg] by the distilled
water density coefficient [μl/mg], which depends on
temperature and pressure according to the following
table:
Temperature [°C]
95.0
20 1.0028
21 1.0030
22 1.0032
23 1.0034
24 1.0037
25 1.0039
If the average aspirated volume exceeds the per missible
value, the following should be done:
• Remove the pipetting pushbutton, (Fig. 4A),
Warning: The pipetting pushbutton consists of 2 parts:
the knob (Fig. 1A2) and the pushbutton (Fig. 1A1). After
removal of the pushbutton, both parts are separated.
• Holding the volume setting knob to protect it against
rotation, insert the calibration key into the cuts of the
calibration screw, (Fig. 4B),
11
Volume
change DV
Preset Permissible for full turn
volume volumes of the
[μl] [μl] calibration key
[μl]
(24 increments)
0.2 0.176 - 0.224 0.06
0.5 0.48 - 0.52 0.33
2 1.94 - 2.06 0.63
5 4.875 - 5.125 2.50
10 9.84 - 10.16 2.50
20 19.76 - 20.24 6.30
50 49.5 - 50.5 6.30
100 98.4 - 101.6 25.00
500 494 - 506 125.00
1000 975 - 1025 250.00
Pressure [kPa]
101.3 105.0
1.0029 1.0029
1.0031 1.0031
1.0033 1.0033
1.0035 1.0036
1.0038 1.0038
1.0040 1.0040
• Turn the key clockwise to reduce the aspirated volume,
or counter-clockwise to increase the volume, (Fig. 4C).
One full turn of the calibration key changes the pipette
aspiration volume by the amount given in the table.
• Take out the key and fix the pipetting pushbutton (Fig. 4D).
The pipetting pushbutton should be fixed by placing the
knob on the arbor first (Fig. 1A2) and then the pushbutton
(Fig. 1A1).
Determine the average aspirated volume. The average
volume should be within the permissible range given in
the table. If the volume exceeds the values stated, the
recalibration procedure should be repeated.
When pipetting liquids with physical properties consi de -
rably different from those of water, follow the rules given
in section 5.
More information on calibration procedure can be found
on www.htl.com.pl

9 - TROUBLESHOOTING

If you notice an improper pipette operation identify the
cause and eliminate the fault. To do this, follow the
instruction in the sequence provided. Replacement of
parts should be required only occasionally, and should
not occur under normal pipette use.
Droplets of liquid remain in the pipette tip.
• The tip is emptied too fast.
Decrease the speed of pressing the pipette push-
button.
• The tip wettability has increased due to extensive use.
Replace the tip with a new one.
Droplets of air appear in the liquid aspirated into the tip.
• The pipette tip immersion is too shallow.
Immerse the tip deeper according to the instructions.
• The pipette tip is incorrectly pressed onto the pipette
shaft.
Press the pipette firmly.
• The tip is damaged or worn out due to extensive use.
Replace the tip with a new one.
The pipette incorrectly aspirates the liquid or liquid
drops out from the tip.
• The pipette tip is incorrectly pressed onto the pipette
shaft.
ENGLISH
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