Recommendations; Recalibration - HTL DISCOVERY Comfort Mode D'emploi

Tips 5000
These tips are used for volumes between 500 μl and
5000 μl. They are used with the DV5000 and the D5000
models, which are equipped with the white pushbutton.
Tips 10000
These tips are used for volumes between 1000 μl and
10000 μl. They are used with the DV10000 and the
D10000 models, which are equipped with the white
pushbutton.

7 - RECOMMENDATIONS

Observing the following recommendations will ensure
maximum possible accuracy and precision of liquid sam-
pling.
• When pipetting the pipette should have the volume set-
ting locked with the locking ring in the upper position.
• Make sure to operate the pipette slowly and smoothly.
• The depth of immersion in the sample liquid should be
the minimum necessary and should remain constant
during aspiration.
• The pipette should be held in a vertical position.
• Change the tip when volume setting is chan ged or when
a different liquid is to be aspirated.
• Change the tip if a droplet remains on the end of the tip
from the previous pipetting operation.
• Each new tip should be pre-rinsed with the liquid to be
pipetted.
• Liquid should never enter the pipette shaft. To pre vent
this:
- Press and release the pushbutton slowly and smoothly.
- Never turn the pipette upside down.
- Never lay the pipette on its side when there is liquid in
the tip.
• Never force the volume setting beyond its recommend-
ed limits.
• When pipetting liquids with temperatures different from
the ambient temperature, it is reco mmended to pre-
rinse the tip several times before use.
• Do not pipette liquids with temperatures above 70°C.
• When pipetting acids or corrosive solutions which emit
vapours, it is recommended to disassemble the shaft
and to rinse the piston and seal with distilled water after
finishing the pipetting operation.
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8 - RECALIBRATION

HTL pipettes are calibrated by gravimetric me thod, using
HTL tips and distilled water, at the temperature 20±1°C,
according to EN ISO 8655 standard.
If during pipette operation you find that the accuracy
error (the difference between the real aspirated volume
and the preset volume) exceeds the permissible value
given in the table in section 1, the pipette recalibration
procedure should be carried out.
Before starting the recalibration it is necessary to check
whether the following requirements have been fulfilled
during error determination:
• the ambient temperature, and the temperature of the
pipette, tips and water was identical
• the density of the liquid used is close to that of distilled
water
• a balance with appropriate sensitivity has been used.
Volume checked [μl]
0.1 - 10
10 - 100
> 100
• mg/μl conversion factor has been taken into ac count
• the requirements given in sections 3 and 7 have been
fulfilled
If the above conditions are ensured and the ac cu racy
error for selected volume given in section 1 exceeds the
permissible value, the pipette recalibration procedure
should be carried out.
The recalibration can be performed within one full
turn of the key to the right or to the left only.
Recalibration conditions:
• Ambient temperature and the temperature of the pi pet -
te, tips and liquid should be within the range 20-25°C
and stabilised during weighing within ±0.5°C
• Measurements should be conducted using distilled
water
• Balance sensitivity should be suitable for the vo lume to
be controlled
Recalibration procedure:
• Set the dose volume depending on the pipette volume
according to the following table:
ENGLISH
Balance sensitivity [mg]
≤ 0.001
≤ 0.01
≤ 0.1
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