Aspirating And Dispensing Liquids; Dv2 D2 - HTL DISCOVERY Comfort Mode D'emploi

Pipettes DV2, D2
Red figures at the bottom = 1/100 μl
Increment = 0.002 μl
Pipettes DV10, DV20, D10, D20
Red figures at the bottom = 1/10 μl
Increment = 0.02 μl
Pipettes DV50, DV100, DV200,
DV250, D50, D100, D200, D250
Black figures only = 1 μl
Increment = 0.2 μl
Pipettes DV1000, DV5000, D1000, D5000
Red figures at the top = ml
Increment =
Pipettes DV10000, D10000
Red figures at the top = ml
Increment = 20 μl
The volume of the pipette is set by the knob in the pipet-
ting pushbutton (Fig. 1A2) or by the black adjustment
knob (Fig. 1B). The volume adjustment can be performed
when the locking ring is set in the lower position (Fig.5A).
When the desired volume is selected, the locking ring should
be set in the upper position (Fig.5B). To attain the maximum
accuracy, set volume must be approached from a higher
value by diminishing counter readings.
• If the desired volume is lower than set volume shown
by the counter, the operator should turn the pipetting
pushbutton (Fig. 1A2) or the black adjustment knob
(Fig. 1B) to the direction diminishing counter readings
to the required volume. Before achieving the required
volume slowly rotate the knob and observe carefully
diminishing reading to avoid accidentally passing the
setting value.
• If the desired volume is higher than set volume shown by
the counter, the operator should turn the pipetting push-
button (Fig. 1A2) or the black adjustment knob (Fig. 1B)
increasing the value until the lower figure wheel comes
5
1/3 of a turn beyond the required setting and then slowly
backward until the setting reaches the desired volume.
Make sure not to pass the setting value.
If the knob is accidentally turned too far, the process
must be repeated. The desired volume must always be
set from the higher value in the order of decreasing value.
Following volume adjustment, set the locking ring into the
upper position, thus locking the knob and preventing
accidental volume change.

3 - ASPIRATING AND DISPENSING LIQUIDS

Place a tip on the shaft of the pipette. See Section 6 for
the appropriate tip. Press the tip on firmly using a slight
twisting motion. This will ensure an airtight seal.
Important: Never aspirate liquids without a tip attached.
Aspiration
Press the pushbutton to the first positive stop, (Fig. 2A).
Holding the pipette vertically, immerse the tip into the
2 μl 10 μl
sample liquid. The depth to which the tip is immersed in
the sample liquid depends on the model.
DV20, DV50, DV100, D20, D50, D100
Release the pushbutton slowly and smoothly to aspirate
the sample, (Fig. 2B). Wait one second and then with-
draw the tip from the liquid. When the pipette tip is
immersed not as deeply as the recommended depth or
when the pipetting pushbutton is rapidly released air
may enter the disposable tip.
Avoid touching the orifice of the tip.
Dispensing
• Place the end of the tip against the inside wall of the
vessel at an angle of 10 to 40 degrees.
• Press the pushbutton smoothly to the first stop, (Fig. 2C).
Wait one second.
Model
DV2, D2
DV10, D10
DV200, DV250, DV1000,
D200, D250, D1000
DV5000, D5000
DV10000, D10000
ENGLISH
Immersion depth (mm)
≤ 1
≤ 1
2 ÷ 3
2 ÷ 3
3 ÷ 6
5 ÷ 7
6