3B 1012852 Mode D'emploi page 3

English
Scope of Supply
The model 'Mini' contains the following items:
• E lectrophoresis chamber with corrosion resistant platinum electrodes and high-quality connections for plus and
minus pole
• Safety lid with attached power cords
• 2 combs, 1.5mm thick, 12 teeth each
• Instruction manual
Safety Instructions
• Please read the instruction manual carefully before using the electrophoresis system.
• Use only CE marked power supplies.
• Always disconnect the electrophoresis chamber from the power supply before removing the safety lid.
• Do not put the safety lid down on wet surfaces.
• Always disconnect the electrophoresis chamber from the power supply when not in use.
• The chamber is provided for a max. current and voltage. Do not exceed 150 mA, 120 V.
• Please mind the maximum filling level for the electrophoresis buffer.
Technical Properties
The horizontal electrophoresis system 'Mini' has been designed in such a way that allows the user to cast and run the gel
directly in a single chamber. No additional casting equipment, such as grease or seal is required to prepare the gel tray for
casting the gel.
The chamber's bottom is UV-permeable, thus the gel run can easily be documented in case of using DNA-fluorescent dyes. Self
evidently, the gel can also be removed from the chamber for staining and documentation after the gel run.
Please use only UV light with a wave length of 300nm or higher for all applications, as hard UV light (<300 nm) damages
the gel chamber and the nucleic acids.
If you need only short runs are needed, you can use both combs which then gives twice as many slots for samples.
Warranty
The manufacturer guarantees that the electrophoresis chamber was completely tested before delivery and that it complies
with the applicable safety regulations.
Please check the delivery immediately upon receipt for completeness and possible damage in transit. If anything is faulty or
damaged, please contact 3B Scientific immediately.
The manufacturer gives a 24-month warranty on the product conditional on the chamber being used according to the instruc-
tion manual. Claims for replacement or repair are not valid if physical abuse is the reason for the damage. Any liability for
conseqential damages resulting from the use of the electrophoresis chamber is hereby excluded. The manufacturer's liability
for intent and gross negligence or for damages resulting from injury to life, body or health is unaffected by that.
Our experience shows the two platinum electrodes last for many years if the product is used properly. Any Damage occur-
ring is usually due to mechanical errors (e. g. by washing-up brushes) or contact with so-called "platinum poisons" such
as phosphorus, boron and heavy metals (lead, zinc, etc.). For this reason, the platinum electrodes are excluded from the
manufacturer's warranty.
In order to introduce developments promptly, the manufacturer reserves the right to change specifications and small visual
details without prior notice.
General Instructions
Casting of agarose gels
1. Please wear protective clothing, goggles and gloves for heating the agarose solution.
2. Remove the safety lid of the electrophoresis chamber carefully.
3. F or the preparation of the gel solution, only suitable agaroses and electrophoresis buffers should be used. For preparing
the agarose solution, weigh a suitable amount of agarose in a small Erlenmeyer flask, add an appropriate amount of elec-
trophoresis buffer and a stir bar (if heating with heating stirrers). Note the weight of the filled flask so that any loss during
heating can be compensated for by the addition of distilled H2O. Thus, the gel solution will have the agarose concentration
Electrophoresis Chamber 'Mini'
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