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Special attention and supervision by adults is necessary, when the scalpel is used to cut objects. Whenever you do not
use the scalpel, wrap it up, and store it in a secure place.
a) Preparation of short-lived or "wet" objects.
(for example tissue samples like parts of plants)
1.
Use the scalpel to cut the part of the plant as thin as possible.
2.
Place the specimen onto the microscope slide.
3.
Use the pipette to place a drop of water onto the microscope slide.
4.
Cautiously place the cover glass on top, so that the water spreads.
5.
The cover glass should be placed as flat as possible, to avoid bubbles. Caution: The thin cover glass will break when
the pressure is too strong.
6.
Carefully dab up eventually escaped water.
7.
Now place the specimen carefully under the microscope (cover glass towards the top), and watch it then.
8.
Use labels to mark your objects.
Maybe you prefer to exchange step two and three, that is, you first put the drop of water onto the microscope slide, and then
the specimen, before adding the cover glass. Experiment with both methods to find the best one for your object. (Keep in
mind that they are short-lived specimen.)
Tip:
The microscope slides can be washed carefully, and then used again. The cover glasses are extremely thin and cannot be
reused. Dispose of them carefully after use.
b) Making simple long-term specimen
Proceed the same way as above, only try to use transparent glue instead of water to keep the object and the cover glass on
the microscope slide. Let the glue dye before you look at the object. Use the labels to mark your specimen.
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