General Description; Theory Of Operation - Bibby Scientific Stuart SHM1 Manuel D'utilisation

Homogenisers
SHM1 & SHM2
Instructions for use
Introduction
Thank you for purchasing this piece of Stuart
equipment. To get the best performance from
the equipment and for your own safety, please
read these instructions carefully before use.
Before discarding the packaging check that all
parts are present and correct.
This equipment is designed to operate under the
following conditions:
 For indoor use only
 Use in a well ventilated area
 Ambient temperature range +5ºC to +40ºC
 Altitude to 2000m
Relative humidity not exceeding 80%

Mains supply fluctuation not exceeding 10%

Over-voltage category II IEC60364-4-443

Pollution degree 2

If the equipment is not used in the manner
described in this manual and with accessories
other than those recommended by Stuart, the
protection provided may be impaired.
Safety Advice Before Use
 Do not allow the motor to be submerged in
any liquid.
 Do not operate the product if it has been
damaged in any way.
 Do not use attachments not recommended
by Stuart.
 Do not attempt to stop movement by hand.
 Do not attempt to modify any part of the
product.
 Keep this product away from heated
surfaces.
 Do not run the generator probes in air, only
in the presence of fluid.

General Description

The Stuart SHM1 and SHM2 tissue homogenisers
are variable speed, hand-held or retort-stand
mounted homogenisers. They combine a
high-speed, high-torque motor with a choice of
autoclavable rotor-stator generator probes. The
model SHM1 tissue homogeniser is suitable for
most laboratory applications such as tissue
homogenisation, production of emulsions and
suspensions, cell disruption and protein
extraction. It is lightweight and can be hand-held
or mounted to a retort stand for lengthier
processing. The SHM2 is a powerful, versatile and
durable homogeniser that can handle virtually
any processing application, making it ideal for
tougher samples and larger volumes.
The SHM1 is supplied with a post clamp assembly
for mounting it to a retort stand. A retort stand
assembly, catalogue number SHM/STAND, is
available as an accessory for both models. Both
models are also supplied with a tool kit for
dismantling the rotor-stator generator probes for
easy cleaning.

Theory of Operation

The rotor shaft part of the probe is coupled
directly to the drive motor of the homogeniser.
When attached, the rotor shaft can spin up to
35,000rpm; this makes up the rotor part of the
rotor-stator generator probe. The tube/collar
assembly which fits over the rotor shaft is
attached to the motor housing but does not spin.
This is the stator part of the rotor-stator
generator probe.
As the rotor knife spins within the tube/collar
assembly, it creates a pumping action, pulling the
sample into the open end of the probe and
forcing it out through the windows in the tube.
This sets up a shearing action, reducing the
particle size of the sample. The speed differential
between the rapidly moving portion and the
relatively stationary portion of the sample sets up
a second force called cavitation which pulls the
sample apart, further reducing the particle size.