Thermo Scientific BioCane Serie Mode D'emploi page 11

Thermo Scientific
Operation
Section 3
To avoid damage to your cryogenic storage vessel which may result in
premature vacuum loss, it is important that the following procedure be
used during the addition of liquid nitrogen to a warm vessel:
1. Add only a small amount of liquid nitrogen (2-10 liters) to new or
warm vessels.
2. Allow this small amount of liquid nitrogen to sit in the covered vessel
for a minimum of 2 hours. This will limit stress caused by the sudden
temperature change associated with adding liquid nitrogen to a warm
vessel.
3. Add an additional 2-10 liters LN2 to vessel.
4. Allow vessel to sit for 48 hours and monitor liquid nitrogen
consumption.
5. Fill vessel as desired. Remember to allow for displacement of liquid
nitrogen when cannisters and canes are inserted.
6. Insert and remove cannisters slowly. Allow liquid nitrogen to run out
of cannisters.
Caution Never overfill liquid nitrogen vessels.
When inserting or removing cans, be careful not to come in contact with
the neck tube area of the vessel. Remove or insert cans slowly, in a
vertical manner. Scratches on the neck tube area can cause premature
vacuum failure.
Do not spill liquid nitrogen on vacuum port - this can cause vacuum
s
failure.
Cryobiological Storage Vessel 3-1