Adjusting Transmitted-Light Phase Contrast Or Transmitted-Light Darkfield - Zeiss Primo Star Mode D'emploi

Carl Zeiss
Fig. 22 Inserting the slider
Fig. 23 Centering the phase stop
Caution: Risk of pinched fingers
If the slider Ph 1 / H / Ph 2 is used, your fingers might be pinched between the slider and the
control knobs of the microscope stage. This risk can be prevented by removing the slotted
screw (on the underside of the condenser) from the middle bore hole and screwing it into the
bore hole to the left or right of it (depending on whether the stage is operated from the right
or from the left).
28
Start-Up and Operation
3.4.5
The phase contrast method on the
Primo Star microscope requires a
stand with Full-Köhler equipment.
• First, adjust the microscope as you do for
brightfield.
• Turn the nosepiece to position the phase-
contrast objective (for Ph 1, Ph 2 or Ph 3) into
the light path.
• Open the luminous-field diaphragm (Fig. 22/3)
on the stand and open the aperture diaphragm
by means of the control lever (Fig. 22/1) on the
Abbe condenser (Fig. 22/2).
• Insert the slider fitting the objective used, with
one (Fig. 22/6) or two (Fig. 22/9) phase contrast
positions (Ph).
Slider
−
Remove the screw (Fig. 22/7). Push the slider
from the left into the Abbe condenser and
screw in the screw (Fig. 22/7) again.
− Slider Ph 1 / H / Ph 2 (Fig. 22/9):
Unscrew the handle (Fig. 22/8) at the right side.
Push the slider (Fig. 22/9) from the left into the
Abbe condenser (the inscriptions Ph 1 and Ph 2
are readable upright and laterally correct from
the front). Screw in the handle again.
Primo Star / Primo Star iLED
Adjusting transmitted-light phase
contrast or transmitted-light
darkfield
Ph 1, Ph 2 or Ph 3
M60-2-0011 v 08/2008
(Fig. 22/6):