Siemens IMMULITE 2000 Mode D'emploi page 3

the reaction tube and signal is generated
in proportion to the bound enzyme.
Incubation Cycles: 1 × 30 minutes
Time to First Result: 35 minutes
Specimen Collection
Patient must be fasting and at complete
rest 30 minutes before blood collection.
The use of an ultracentrifuge is
recommended to clear lipemic samples.
Hemolyzed samples may indicate
mistreatment of a specimen before receipt
by the laboratory; hence the results should
be interpreted with caution.
Centrifuging serum samples before a
complete clot forms may result in the
presence of fibrin. To prevent erroneous
results due to the presence of fibrin,
ensure that complete clot formation has
taken place prior to centrifugation of
samples. Some samples, particularly
those from patients receiving
anticoagulant therapy, may require
increased clotting time.
Blood collection tubes from different
manufacturers may yield differing values,
depending on materials and additives,
including gel or physical barriers, clot
activators and/or anticoagulants.
IMMULITE 2000 Growth Hormone has not
been tested with all possible variations of
tube types. Consult the section on
Alternate Sample Types for details on
tubes that have been tested.
Volume Required: 25 µL serum
Storage: Refrigerate immediately. Stable
at 2–8°C for 8 hours,
(aliquotted) at –20°C. Avoid repeated
thawing and freezing.
Warnings and Precautions
For in vitro diagnostic use.
CAUTION: This device contains material
of animal origin and should be handled as
a potential carrier and transmitter of
disease.
IMMULITE 2000 Growth Hormone (hGH) (PIL2KGRH-11, 2018-03-15)
6,7
6,7 or for 2 months
H412 Harmful to aquatic life
with long lasting effects.
P273, P501
Avoid release to the
environment. Dispose of
contents and container in
accordance with all local,
regional, and national
regulations.
Contains: sodium azide;
GH Adjustors
Reagents: Store at 2–8°C. Dispose of in
accordance with applicable laws.
Follow universal precautions, and handle
all components as if capable of
transmitting infectious agents. Source
materials derived from human blood were
tested and found nonreactive for syphilis;
for antibodies to HIV 1 and 2; for
hepatitis B surface antigen; and for
antibodies to hepatitis C.
Sodium azide, at concentrations less than
0.1 g/dL, has been added as a
preservative. On disposal, flush with large
volumes of water to prevent the buildup of
potentially explosive metal azides in lead
and copper plumbing.
Chemiluminescent Substrate: Avoid
contamination and exposure to direct
sunlight. (See insert.)
Water: Use distilled or deionized water.
Materials Supplied
Components are a matched set. Labels on
the inside box are needed for the assay.
GH Bead Pack (L2GRH12)
With barcode. 200 beads, coated with
murine monoclonal anti-hGH antibody.
Stable at 2–8°C until expiration date.
L2KGRH2: 1 pack
GH Reagent Wedge (L2GRHA2)
With barcode. 11.5 mL alkaline
phosphatase (bovine calf intestine)
conjugated to rabbit polyclonal anti-hGH
antibody in buffer. Stable at 2–8°C until
expiration date.
L2KGRH2: 1 wedge
Before use, tear off the top of the label at
the perforations, without damaging the
barcode. Remove the foil seal from the top
of wedge; snap the sliding cover down into
the ramps on the reagent lid.
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