Setup And Use - VWR VisiScope 400 Serie Manuel D'utilisation

SETUP AND USE

Installing the phase contrast slider
1. Insert the slider into the illumination system, printed face up.
2. Pull the slider into the desired position, to the click stop.
3. When in phase contrast observation, keep the aperture diaphragm adjustment lever on the "O" (open)
position.
Centering the ring
► Usually this operation is not needed. If necessary, please proceed with the following steps:
1. Place a specimen on the stage and focus it.
2. Take out the eyepiece from the tube without the dioptric adjustment, and replace it with the centering
telescope (CT).
3. Check that the phase ring and the objective correspond, and that both are steadily set on a click stop.
4. Use the CT to focus on the light ring's image (5) and the phase contrast ring's image (2). If the light ring's
image is not sharp, adjust the CT's eyepiece until you can see a clear image of the light ring (2).
5. Adjust the bolts of the two centering holes in the phase contrast slider using a screwdriver until the light
ring center and the phase contrast ring center coincide.
6. The 10X and the 20X phase contrast objectives use the same ring on the phase contrast slider. The co-
incidence of the light ring center and the phase contrast center must be verified with both objectives.
► If the light ring is centered incorrectly, the contrast will be severely impaired.
► The phase ring may need recentering during and after observation of very thick specimens.
► The phase ring may show an apparent misalignment if the cover glass is not flat.
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