Identifying The Components - VWR VisiScope 400 Serie Manuel D'utilisation

COLOR FILTER
In
Out
Using the aperture diaphragm
When in bright field observation, the aperture diaphragm controls the numerical aperture of the illumination
system. When the numerical aperture of the objective and the aperture of the illumination system match, the
highest resolution is achieved.
To identify the aperture diaphragm, the eyepiece can be removed or the centering telescope can be used.
The aperture can be changed by moving the aperture adjustment lever. ((1)is the image of the aperture dia-
phragm, (2) is the edge of the objective).
Generally, when observing a fully chromatic specimen, you need to set the size of the condenser to 70-80%
of the aperture of the objective. When observing unstained samples (e.g. bacteria), start from 70% and slowly
turn the aperture diaphragm lever clockwise.

IDENTIFYING THE COMPONENTS

Phase contrast objectives
The magnifications of the optional phase contrast objectives are: 10X and 20X. These objectives are marked
"PH". Mount the objectives on the turret using the same procedures as the standard objectives
Phase contrast slider
Adjustable phase slider.
● The light ring is pre-centered when the microscope leaves the
factory. It should therefore need no further adjustment. If a re-
centering is needed, it can be performed via the two side bolts.
● The 4X/10X light ring (1) must be used with 4X and 10X phase
contrast objectives, the 20x/40x light ring (2) with the 20x and
40x and the opening (3) is used for bright field.
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Single contrast color filter (green) used for phase contrast microscopy
Color temperature compensation color filter blue used for bright field obser-
vation and microphotography
Handbücher-Suchmachiene
USE
1
2
13
70-80%
70-
80%
30-20%
30-
20%
1
2
3