Celestron 44100 Manuel De L'utilisateur page 16

Viewing a Specimen
Your instrument is provided with a mechanical stage with a stage holder clamp and directional knobs –see figure 4g and 4h.
A vernier scale on both axes allows the exact marking and replication of an object in the field of view that the user may want to
come back to.
1. Use the clamp lever to open the clamping arm of the stage holder clamp.
2. Place a specimen slide (3" size) inside the holder and gently close the clamping arm against the slide.
3. Use the stage movement knobs (see Figure 4h) to position the specimen over the opening in the stage (5).
You are now ready to focus and view the specimen, but first you must take some precautions so as not to damage the speci-
men slide or objective lens. Your microscope is equipped with a safety rack stop (9) which allows you to regulate the range of
travel on the focuser. This assures that the objective lens will not accidentally come into contact with the specimen slide, break-
ing the slide or scratching the objective lens. To adjust the travel on the focus mechanism:
4. Raise the objective lens away from the specimen stage (5) by rotating the coarse focus knob (3) backwards (clockwise).
5. Unthread the safety rack stop screw (9) about 2/3 of the way.
6. Turn the nosepiece (12) until the 40x lens is over the specimen.
7. Lower the objective lens over the slide until the tip of the lens is slightly above the specimen slide. (Be careful not to
touch the objective lens to the slide).
8. Thread the safety screw (9) upward until it stops against the focus mechanism.
9. Rotate the knurled locking nut upward to lock the safety screw in place.
Figure 4g
Tip: To position the specimen directly under the objective lens, close the opening on the iris diaphragm (see Figure 4h) until it
is almost completely closed. You should see a small beam of light projected onto the specimen slide. Now simply use the stage
movement knobs to move the specimen directly inside the beam of light.
10. Use the nosepiece (12) to rotate the objective lenses (4) until the 4x objective is directly over the specimen. Always start
with the lowest power objective and work your way up to higher powers.
11. Look through the eyepiece while turning the coarse focus knob (3) until the specimen comes into view. You may need to
adjust the stage knobs (Figure 4h) slightly to center the specimen in the field of view.
Warning: When focusing, be careful not to raise the specimen stage so high that the specimen slide
touches the objective lens. Not only can you break your slide but you may scratch the objective lens.
12. Finally, adjust the fine focus knob (11) until you reach the sharpest focus for your eye.
Warning: Never rotate both of the fine focus knobs at the same time in opposite directions or the focusing
mechanism may get damaged.
I ENGLISH
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Abbe Condenser
Iris Diaphragm
Movement Knobs
Stage
Figure 4h