Clarifying Filtration; Sterile Filtration - Sartorius Stedim Biotech 16306 Mode D'emploi

5. Clarifying Filtration

5.1 Assemble the filter holder and insert the membrane
filter as described in section 4.
5.2 Pour the liquid to be filtered into the glass
funnel (see Fig. 9) and switch on the vacuum pump.
Important Note:
If you are additionally using a prefilter, switch the va-
cuum pump on before filling the funnel with the liquid
to be filtered. (Otherwise, the prefilter may float to the
top of the liquid.)
5.3 Filtration is completed when all the liquid has passed
through the membrane filter. Disconnect the vacuum
source from the filter holder by pulling the hose off
the hose nipple on the suction flask (if the water jet
pump is used; see Fig.10). If a Woulff's bottle and a
vacuum pump have been used, switch off the pump
and release vacuum via the Woulff's bottle (see Fig. 11).
5.4 Now remove the filter holder and stopper from the
suction flask and transfer the filtrate to another con-
tainer or leave it in the suction flask.

6. Sterile Filtration

(This is possible only with the filter support and
PTFE-coated screen, and not the glass filter support.)
6.1 Assemble the filter holder and insert the mem brane
filter as described in section 4; however, do not con-
nect the filter holder to a vacuum source or a Woulff's
bottle.
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