Clarifying Filtration - Sartorius Stedim Biotech 16309 Instructions D'utilisation

If you are using an electric pump as a
source of vacuum, you must intercon-
nect a Woulff's bottle between the filtrate
flask and the pump to prevent any filtrate
overflow from entering the pump. When
used with a water jet pump, Midisart 2000
prevents water from flowing back into the
filtrate flask (Fig. 8).
If the filtrate flow rate decreases, we recom-
mend that you use compressed air
to drain any remaining water out of the
Midisart 2000 (in the direction opposite
to filtration), or that you allow the
Midisart 2000 to dry overnight in a
drying oven at 105 °C.
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5. Clarifying Filtration

5.1 Assemble the filter holder and insert the
membrane filter as described in section 4.
5.2 Pour the filtration medium into
the glass funnel (Fig. 9), and turn on the
vacuum pump.
Important Note
If you are additionally using a prefilter for
your filtration run, we recommend that you
turn on the vacuum pump before pouring
the filtration medium into the glass funnel
(otherwise, the prefilter could float to the
top).
5.3 The filtration run is completed when the
filtration medium is no longer visible on the
membrane filter. At this point, disconnect
the source of vacuum from the filter holder
(if you are using a water jet pump, just
detach the hose from the nipple on the
suction flask). If you are using a Woulff's
bottle and a vacuum pump, turn off the
pump and release the pressure in the
Woulff's bottle.
5.4 Remove the glass funnel.
5.5 There are two possibilities for removing
the filtrate:
a) After detaching the vacuum hose and
removing the filter support, you can pour
out the filtrate from the side connector
(Fig. 10).
b) After removing the glass base, you can
pour out the filtrate (Figs. 11/12).