Optika Italy B-380 Serie Manuel D'instructions page 22

10.10 Use of oil immersion objective
1. Focus the specimen with a low power objective.
2. Lower the stage.
3. Put a drop of oil (provided) on the area of the specimen to be
observed. (Fig. 21)
• Make sure that there are no oil bubbles. Air bubbles in
the oil damage the image quality.
• To check for bubbles: remove an eyepiece, fully open the ap-
erture diaphragm and observe the objective exit pupil. (The
pupil must be circular and bright).
• To remove the bubbles, gently move the nosepiece to the
right and left to move the immersion objective a few times
and allow the air bubbles to move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and obtain an
optimal focus using the fine focus knob.
6. After use, gently remove the oil with a soft paper towel or a
lightly moistened optic paper with a mixture of ethyl ether
(70%) and absolute ethyl alcohol (30%).
• The immersion oil, if not immediately cleaned, could
crystallize creating a glass-like layer. In this situation
the observation of the specimen would be difficult (even
not impossible) due to the presence of an additional
thickness on the objective.
10.11 Use of ALC system
• The ALC system does NOT allow a camera to be mount-
ed. The photo port is closed with a cap engraved with
"ALC", and the cap is glued to prevent the photo port
from being used.
• If you need to use a camera: remove an eyepiece and in-
sert the projection lens into the empty eyepiece sleeve.
1. Adjust the desired brightness through the eyepieces using
the light intensity dial (chapter 10.1).
2. Press the ALC button
light on the microscope will turn off for some seconds, then
will turn on again; ALC button will light up in blue showing that
ALC system is active.
• The settings could not be working when the light intensi-
ty is too low or too high. This is not a defect.
3. Now the system will automatically adapt the brightness to the
eyepieces when an objective is changed, when the aperture
diaphragm is used or when another specimen is placed on
the stage.
4. Pressing the ALC button again, the ALC system will be dis-
abled.
• When ALC system is active the light intensity dial is not
active.
10.12 Use of the polarizer (optional)
1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the polarizer until the
darkest position is achieved.
3. Once the dark is achieved ("extinction" or "Crossed Nicol"
position) it is possible to begin the observation.
to store this setting (Fig. 22). The
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