Phase Contrast Slider; Centering The Phase Ring - Optika Italy IM Serie Manuel D'utilisation

9.11.1 Phase contrast slider

Adjustable phase slider.
• The phase ring is pre-centered when the micro-
scope leaves the factory. It should therefore need
no further adjustment. If a recentering is needed,
it can be performed using the two side bolts.
• The 4X/10X phase ring ① must be used with 4X
and 10X objectives, the 20x/40x phase ring ②
with the 20x and 40x and the opening ③ is used
for bright field. (Fig. 35)

9.11.2 Centering the phase ring

Usually this operation is not needed. If neces-
sary, please proceed with the following steps:
1. Place a specimen on the stage and focus it.
2. Take out the eyepiece from the eyepiece tube
without the dioptric adjustment, and replace it
with the centering telescope (CT). (Fig. 36)
3. Check that the phase ring and the objective cor-
respond, and that both are steadily set on a click
stop.
4. Use the CT to focus the condenser phase ring
(bright) ① and the objective phase ring (dark)
image ②. If the bright phase ring's image is not
sharp, adjust the CT head eyepiece until you can
see a clear image of the phase ring.
5. Adjust the bolts of the two centering holes in the
phase contrast slider using a screwdriver until
the bright phase ring and the dark phase contrast
ring coincide.
6. The 4x and the 10X phase contrast objectives
use the same ring on the phase contrast slider.
The coincidence of the phase ring center and the
phase contrast center must be verified with both
objectives. (Fig. 37-38)
• If the phase ring is centered incorrectly, the
contrast will be severely impaired.
• The phase ring may need recentering during
and after observation of very thick specimens.
• The phase ring may show an apparent mis-
alignment if the specimen is not flat.
①
①
②
Page 21
② ③
Fig. 35
Fig. 36
Fig. 37
Fig. 38