Sartorius Vivapore 5 Mode D'emploi page 2

Vivapore 5, 10|20 ml C
Directions for Use
Intended only for in-vitro diagnosis
Vivapore Concentrators offer exceptional ease of use
and flexibility for the concentration and|or purifica-
tion of macromolecules from dilute solutions.
Depending on configuration and reservoir volume of
the Vivapore selected, starting volumes may vary from
3 to 20 ml and up to 750 concentration may be
achieved.
Operation
1. Concentrating macromolecules
a. Insert Vivapore 5 or 10| 20 into stand.
b. Pipette sample through aperture at the top of the
device. Device can be left unattended until desired
concentration is achieved. Solvent and micromol-
ecules are pulled through the membrane by a high-
capacity absorbent; a dead stop feature prevents the
sample from concentrating to dryness.
c. Once the desired volume is achieved, the concen-
trate is withdrawn using a Pasteur, thin plastic or
gel loader type pipette. The sample is now ready for
further analysis.
Please note, the graduated scale on the device is for
indication only.
2 |
2. 20 ml capacity expansion for
the VP10| 20
Sample capacity of the VP10| 20 can be increased
to 20 ml by use of the ancillary reservoir. First pipette
10 ml into the Vivapore, then lock the ancillary
reservoir to the top opening and add up to an addi-
tional 10 ml of sample.
3. Desalting with Vivapore 10|20
Reservoir expansion or desalting by constant vol-
ume diafiltration. VP10 |20 offer a unique ancillary
reservoir feature that allows reservoir expansion or
one-step purification and concentration of protein
samples.
In this procedure, new buffer solution replaces the
original solvent at a rate equal to the speed of filtra-
tion. With a fresh buffer solution volume that is five
times greater than the sample in the concentrator,
99% of salts will be removed.
A. Pipette up to 2 ml of sample to be purified
through aperture at the top of the device.
B. Firmly seat a 10 ml plastic pipette or the ancillary
reservoir on the conical entry port of the concentra-
tor. Fill with up to 5 times the volume in the concen-
trator using deionized water or fresh buffer solution.
Device can be left unattended whilst the new buffer
replaces the solvent in the original solution. Once
buffer in the pipette is exhausted, the remaining solu-
tion will continue to concentrate to the level desired.
C. Collect purified and concentrated sample with a
pasteur or long gel loader type pipette.