Use Of The Microscope In Polarized Light; Centering The Nosepiece - Optika Italy B-380 Serie Manuel D'instructions

10. Use of the microscope in polarized light

• The system allows observation in Orthoscopy (crossed Nicol) or in Conoscopy (crossed Nicol with
the use of the Bertrand lens).
• For optimal performance in polarized light microscopy, accurate optical adjustments are essential
before beginning the observation.

10.1 Centering the nosepiece

1. Loosen the stage rotation lock ① and rotate the
stage until the graduated scale of the stage ②
and the vernier scale ③ are aligned in the "0"
position. (Fig. 14)
• This operation serves to ensure a standard
reference position for centering the nosepiece.
2. Focus on a recognizable detail ④ in the field of
view by placing it at the centre of the crosshair.
(Fig. 15)
3. By turning the stage, the detail in focus will
describe a circle ⑤. (Fig. 15)
4. Move again the stage on the "0" position and lock
the locking screw ①.
5. Operating on the nosepiece centering screws ⑥
move the detail in diametrically opposite direction
to the described circle. The movement must be
about half the diameter of the circle described.
(Fig. 16)
6. Manually move the specimen and put it again
on the centre of the crossahir. Loosen again the
stage rotation lock and rotate once more the
stage.
7. If the centering has been carried out correctly, by
turning the table the image of the focused detail
does not move with respect to the centre of the
crosshair. If not, repeat the operations described
by 2. to 6. until the perfect coincidence of the
centre of rotation of the stage with the centre of
the crosshair for which the specimen remains in
the centre of the crosshair turning the table.
8. Once centred with the 10x, rotate the nosepiece
to insert in the optical path all the other objectives
and verify the correct centering of the objectives,
acting on the screws of centering the nosepiece
⑥, to make sure that all the objectives are
perfectly centred with respect to the optical axis.
③
②
①
⑥
Page 13
⑤ ④
F ig. 14 F ig. 14
F ig. 15 F ig. 15
⑥
F ig. 16 F ig. 16